Influence of Fibrinogen on Staphylococcus epidermidis Adhesion Can Be Reversed by Tuning Surface Nanotopography
Journal article, 2019

Surface modifications in the nanoscale regime have shown promising potential in the combat against bacterial adhesion and colonization of surfaces. However, detailed knowledge of how the bacteria-substrate interactions occur is still limited. Herein we have used a gradient in nanostructure density on a surface, realized by immobilizing 40 nm sized silicon dioxide nanoparticles with increasing distance on a glass surface, to systematically study the initial attachment of Staphylococcus epidermidis with or without the presence of human fibrinogen. By using a parallel plate laminar flow chamber, we found a near-linear positive correlation between the adhesion of S. epidermidis with increasing nanoparticle density on unmodified (hydrophilic) nanogradients as well as on gradients where polyethylene glycol was immobilized on the surface in-between nanoparticles. However, if the nanostructured gradient was precoated with human fibrinogen the opposite relationship was observed, although the adsorbed amount of fibrinogen was found to be higher on nanostructured than on smooth surfaces. Our results highlight that even minute changes of the nanotopography on a surface can have profound impact on initial attachment of S. epidermidis to biomaterial surfaces and that the presence of nanostructures strongly hampered the cell's ability to bind to adsorbed fibrinogen, possibly due to changes in the orientation or secondary structure of the fibrinogen molecule upon adsorption.

bacterial adhesion

biomaterial associated infection

flow chamber

protein adsorption

nanotopography

nanoparticles

Author

Mats Hulander

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry

Nordisk Institutt for Odontologiske Materialer

Håkon Valen-Rukke

Nordisk Institutt for Odontologiske Materialer

Gustav Sundell

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Martin Andersson Group

Martin Andersson

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Martin Andersson Group

ACS Biomaterial Science and Engineering

2373-9878 (eISSN)

Vol. 5 9 4323-4330

Subject Categories

Materials Chemistry

Other Chemistry Topics

Bioengineering Equipment

DOI

10.1021/acsbiomaterials.9b00450

More information

Latest update

11/11/2019