Doxorubicin-loaded delta inulin conjugates for controlled and targeted drug delivery: Development, characterization, and in vitro evaluation
Journal article, 2019

Delta inulin, also known as microparticulate inulin (MPI), was modified by covalently attaching doxorubicin to its nanostructured surface for use as a targeted drug delivery vehicle. MPI is readily endocytosed by monocytes, macrophages, and dendritic cells and in this study, we sought to utilize this property to develop a system to target anti-cancer drugs to lymphoid organs. We investigated, therefore, whether MPI could be used as a vehicle to deliver doxorubicin selectively, thereby reducing the toxicity of this antibiotic anthracycline drug. Doxorubicin was covalently attached to the surface of MPI using an acid-labile linkage to enable pH-controlled release. The MPI-doxorubicin conjugate was characterized using FTIR and SEM, confirming covalent attachment and indicating doxorubicin coupling had no obvious impact on the physical nanostructure, integrity, and cellular uptake of the MPI particles. To simulate the stability of the MPI-doxorubicin in vivo, it was stored in artificial lysosomal fluid (ALF, pH 4.5). Although the MPI-doxorubicin particles were still visible after 165 days in ALF, 53% of glycosidic bonds in the inulin particles were hydrolyzed within 12 days in ALF, reflected by the release of free glucose into solution. By contrast, the fructosidic bonds were much more stable. Drug release studies of the MPI-doxorubicin in vitro, demonstrated a successful pH-dependent controlled release effect. Confocal laser scanning microscopy studies and flow cytometric analysis confirmed that when incubated with live cells, MPI-doxorubicin was effciently internalized by immune cells. An assay of cell metabolic activity demonstrated that the MPI carrier alone had no toxic effects on RAW 264.7 murine monocyte/macrophage-like cells, but exhibited anti-cancer effects against HCT116 human colon cancer cells. MPI-doxorubicin had a greater anti-cancer cell effect than free doxorubicin, particularly when at lower concentrations, suggesting a drug-sparing effect. This study establishes that MPI can be successfully modified with doxorubicin for chemotherapeutic drug delivery.

Advax

Doxorubicin

Targeted delivery

Intracellular drug release

Anticancer therapy

Inulin

pH sensitive

Author

Lixin Wang

University of South Australia

Yunmei Song

University of South Australia

Ankit Parikh

University of South Australia

Paul Joyce

Chalmers, Physics, Biological Physics

Rosa Chung

University of South Australia

Liang Liu

University of South Australia

Franklin Afinjuomo

University of South Australia

John D. Hayball

University of Adelaide

University of South Australia

Nikolai Petrovsky

Vaxine Pty Ltd

Flinders University

Thomas G. Barclay

University of South Australia

Sanjay Garg

University of South Australia

Pharmaceutics

1999-4923 (ISSN)

Vol. 11 11 581

Subject Categories

Cell Biology

Cell and Molecular Biology

Pharmacology and Toxicology

DOI

10.3390/pharmaceutics11110581

More information

Latest update

1/29/2021