3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human Neuroblastoma Cells
Journal article, 2020

We prepared cellulose nanofibrils-based (CNF), alginate-based and single-walled carbon nanotubes (SWCNT)-based inks for freeform reversible embedding hydrogel (FRESH) 3D bioprinting of conductive scaffolds. The 3D printability of conductive inks was evaluated in terms of their rheological properties. The differentiation of human neuroblastoma cells (SH-SY5Y cell line) was visualized by the confocal microscopy and the scanning electron microscopy techniques. The expression of TUBB3 and Nestin genes was monitored by the RT-qPCR technique. We have demonstrated that the conductive guidelines promote the cell differentiation, regardless of using differentiation factors. It was also shown that the electrical conductivity of the 3D printed scaffolds could be tuned by calcium-induced crosslinking of alginate, and this plays a significant role on neural cell differentiation. Our work provides a protocol for the generation of a realistic in vitro 3D neural model and allows for a better understanding of the pathological mechanisms of neurodegenerative diseases.

3D bioprinting

carbon nanotubes

cellular models

3D cell cultures

conductive scaffold

Author

Matteo Bordoni

IRCCS Mondino Fdn

Erdem Karabulut

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Paul Gatenholm Group

Volodymyr Kuzmenko

Chalmers, Microtechnology and Nanoscience (MC2), Electronics Material and Systems Laboratory

Valentina Fantini

Golgi Cenci Fdn

Universita degli studi di Pavia

Orietta Pansarasa

IRCCS Mondino Fdn

Cristina Cereda

IRCCS Mondino Fdn

Paul Gatenholm

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Paul Gatenholm Group

Cells

2073-4409 (eISSN)

Vol. 9 3 682

Subject Categories

Cell Biology

Neurosciences

Cell and Molecular Biology

DOI

10.3390/cells9030682

PubMed

32168750

More information

Latest update

6/29/2020