Dopamine-functionalized graphene oxide as a high-performance material for biosensing
Journal article, 2020
We describe a nanocomposite material for the electrochemical detection of beta-nicotinamide adenine dinucleotide (NADH), a coenzyme involved in redox reactions of all living cells and in the detection of many organic species by electrochemical biosensors. The composite is made of nanosheets of electrochemically exfoliated graphene oxide (EGO) covalently functionalized with dopamine (DP) molecules. The EGODP material finally obtained is rich of 1,2-dihydroxyphenyl moieties and is able to detect NADH at a particular low potential value with higher sensitivity with respect to pristine EGO. To study the effectiveness of 1,2-dihydroxyphenyl moieties in inducing electrocatalytic oxidation of NADH, we combined standard voltammetric techniques with UV-Vis absorption spectroelectrochemistry, which allowed us to measure the variations in composition occurring at the electrode vertical bar solution interface, i.e. to measure the consumption rate of NADH. Spectroelectrochemical tests performed by polarising the electrode at a fixed potential value were finally used to compare the performance of EGODP with both EGO and EGO-DP blend (MIX) for the detection of NADH. The covalently functionalized EGO (EGODP) shows sensitivity to NADH up to 300 M-1, around 180 % and 140 % better than either pristine EGO or MIX, respectively.
graphene oxide
spectroelectrochemistry
NADH
1,2-dihydroxyphenyl moieties
chemical functionalization
Author
Fabio Vulcano
University of Modena and Reggio Emilia
National Research Council of Italy (CNR)
Alessandro Kovtun
National Research Council of Italy (CNR)
Cristian Bettini
National Research Council of Italy (CNR)
Zhenyuan Xia
National Research Council of Italy (CNR)
Chalmers, Industrial and Materials Science, Materials and manufacture
Andrea Liscio
National Research Council of Italy (CNR)
Fabio Terzi
University of Modena and Reggio Emilia
Aranzazu Heras
Universidad de Burgos
Alvaro Colina
Universidad de Burgos
Barbara Zanfrognini
National Research Council of Italy (CNR)
Manuela Melucci
National Research Council of Italy (CNR)
Vincenzo Palermo
National Research Council of Italy (CNR)
Chalmers, Industrial and Materials Science, Materials and manufacture
Chiara Zanardi
University of Modena and Reggio Emilia
National Research Council of Italy (CNR)
2D Materials
2053-1583 (eISSN)
Vol. 7 2 024007Subject Categories (SSIF 2011)
Analytical Chemistry
Materials Chemistry
DOI
10.1088/2053-1583/ab734f