Dopamine-functionalized graphene oxide as a high-performance material for biosensing
Journal article, 2020

We describe a nanocomposite material for the electrochemical detection of beta-nicotinamide adenine dinucleotide (NADH), a coenzyme involved in redox reactions of all living cells and in the detection of many organic species by electrochemical biosensors. The composite is made of nanosheets of electrochemically exfoliated graphene oxide (EGO) covalently functionalized with dopamine (DP) molecules. The EGODP material finally obtained is rich of 1,2-dihydroxyphenyl moieties and is able to detect NADH at a particular low potential value with higher sensitivity with respect to pristine EGO. To study the effectiveness of 1,2-dihydroxyphenyl moieties in inducing electrocatalytic oxidation of NADH, we combined standard voltammetric techniques with UV-Vis absorption spectroelectrochemistry, which allowed us to measure the variations in composition occurring at the electrode vertical bar solution interface, i.e. to measure the consumption rate of NADH. Spectroelectrochemical tests performed by polarising the electrode at a fixed potential value were finally used to compare the performance of EGODP with both EGO and EGO-DP blend (MIX) for the detection of NADH. The covalently functionalized EGO (EGODP) shows sensitivity to NADH up to 300 M-1, around 180 % and 140 % better than either pristine EGO or MIX, respectively.

NADH

1,2-dihydroxyphenyl moieties

graphene oxide

spectroelectrochemistry

chemical functionalization

Author

Fabio Vulcano

Universita Degli Studi Di Modena E Reggio Emilia

National Research Council of Italy (CNR)

Alessandro Kovtun

National Research Council of Italy (CNR)

Cristian Bettini

National Research Council of Italy (CNR)

Zhenyuan Xia

Chalmers, Industrial and Materials Science, Materials and manufacture

Istituto per la Sintesi Organica e la Fotoreattività

Andrea Liscio

National Research Council of Italy (CNR)

Fabio Terzi

Universita Degli Studi Di Modena E Reggio Emilia

Aranzazu Heras

Universidad de Burgos

Alvaro Colina

Universidad de Burgos

Barbara Zanfrognini

National Research Council of Italy (CNR)

Manuela Melucci

National Research Council of Italy (CNR)

Vincenzo Palermo

Chalmers, Industrial and Materials Science, Materials and manufacture

Istituto per la Sintesi Organica e la Fotoreattività

Chiara Zanardi

National Research Council of Italy (CNR)

Universita Degli Studi Di Modena E Reggio Emilia

2D Materials

2053-1583 (eISSN)

Vol. 7 2 024007

Subject Categories

Analytical Chemistry

Materials Chemistry

DOI

10.1088/2053-1583/ab734f

More information

Latest update

6/30/2020