Melatonin and vitamin E alleviate homocysteine‐induced oxidative injury and apoptosis in endothelial cells
Journal article, 2020

A relationship exists between hyperhomocysteinemia and cardiovascular diseases, although the underlying mechanisms are still incompletely defined. One possibility involves a homocysteine (Hcy)-induced increased oxidative stress. Melatonin (Mel) and vitamin E (vitE) are important anti-oxidants. The main purpose of this study was (1) to compare the effect of treatments with Mel, vitE or both, on Hcy-induced apoptosis in human umbilical vein endothelial cells (HUVECs), and (2) to investigate the underlying mechanisms. Cell proliferation assay was carried out by Water Soluble Tetrazolium-1 (WST-1) assay kit. Apoptotic index was calculated by TUNEL Assay. Anti-oxidant parameters were studied by measurement of reactive oxygen species (ROS) and lipid peroxidation (LPO) levels. mRNA and protein expression levels of apoptotic and anti-apoptotic genes and proteins were studied by quantitative real time polymerase chain reaction (qRT-PCR) and Western blotting experiments respectively. The results showed that treatments with Mel, vitE or Mel + vitE suppressed Hcy-induced cell death, with a higher efficiency for the Mel and Mel + vitE treatments. Our results suggests that the mechanisms by which these anti-oxidants protected endothelial cells include the decrease in ROS and LPO levels, an increase in cell migration, the downregulation of pro-apoptotic proteins Cas 3, Cas 9, Cyt C and Bax and the upregulation of anti-apoptotic protein Bcl 2. Collectively, these results revealed the protective role of vitE and Mel against Hcy-induced cell apoptosis, which may add insight into therapeutic approaches to Hcy-induced damages.

Melatonin

Apoptosis

Hyperhomocysteinemia

Vitamin E

Homocysteine

Author

Gurkan Aykutoglu

Bingöl Üniversitesi

Musa Tartik

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Bingöl Üniversitesi

Ekrem Darendelioglu

Bingöl Üniversitesi

Adnan Ayna

Bingöl Üniversitesi

Giyasettin Baydas

Altinbas Universitesi

Molecular Biology Reports

0301-4851 (ISSN) 1573-4978 (eISSN)

Vol. 47 7 5285-5293

Subject Categories

Biochemistry and Molecular Biology

Immunology

Cell and Molecular Biology

DOI

10.1007/s11033-020-05607-z

PubMed

32592115

More information

Latest update

10/6/2020