Protein Concentrations of Thrombospondin-1, MIP-1β, and S100A8 Suggest the Reflection of a Pregnancy Clock in Mid-Trimester Amniotic Fluid
Journal article, 2020
The development of immunoassays enables more sophisticated studies of the associations between protein concentrations and pregnancy outcomes, allowing early biomarker identification that can improve neonatal outcomes. The aim of this study was to explore associations between selected mid-trimester amniotic fluid proteins and (1) overall gestational duration and (2) spontaneous preterm delivery. A prospective cohort study, including women undergoing mid-trimester transabdominal genetic amniocentesis, was performed in Gothenburg, Sweden, 2008–2016 (n = 1072). A panel of 27 proteins related to inflammation was analyzed using Meso-Scale multiplex technology. Concentrations were adjusted for gestational age at sampling, experimental factors, year of sampling, and covariates (maternal age at sampling, parity (nulliparous/multiparous), smoking at first prenatal visit, and in vitro fertilization). Cox regression analysis of the entire cohort was performed to explore possible associations between protein concentrations and gestational duration. This was followed by Cox regression analysis censored at 259 days or longer, to investigate whether associations were detectable in women with spontaneous preterm delivery (n = 47). Finally, linear regression models were performed to analyze associations between protein concentrations and gestational duration in women with spontaneous onset of labor at term (n = 784). HMG-1, IGFBP-1, IL-18, MIP-1α, MIP-1β, S100A8, and thrombospondin-1 were significantly associated with gestational duration at term, but not preterm. Increased concentrations of thrombospondin-1, MIP-1β, and S100A8, respectively, were significantly associated with decreased gestational duration after the Holm-Bonferroni correction in women with spontaneous onset of labor at term. This adds to the concept of a pregnancy clock, where our findings suggest that such a clock is also reflected in the amniotic fluid at early mid-trimester, but further research is needed to confirm this.
Inflammation
Proteins
Gestational duration
Amniotic fluid
Mid-trimester
Author
Felicia Viklund
Sahlgrenska University Hospital
Stockholm South General Hospital
Maria Hallingström
University of Gothenburg
Sahlgrenska University Hospital
M. Kacerovsky
University Hospital Hradec Kralove
T. Cobo
University of Barcelona
CIBER Enfermedades Raras
Kristin Skogstrand
Statens Serum Institut
David M. Hougaard
Statens Serum Institut
Karin Sävman
Sahlgrenska University Hospital
University of Gothenburg
Ylva Carlsson
Sahlgrenska University Hospital
University of Gothenburg
Panos Tsiartas
University of Gothenburg
Sahlgrenska University Hospital
Julius Juodakis
Sahlgrenska University Hospital
Victoria University of Wellington
Staffan Nilsson
University of Gothenburg
Chalmers, Mathematical Sciences, Applied Mathematics and Statistics
Bo Jacobsson
Sahlgrenska University Hospital
University of Gothenburg
Norwegian Institute of Public Health
Reproductive Sciences
1933-7191 (ISSN) 19337205 (eISSN)
Vol. 27 12 2146-2157Subject Categories
Environmental Health and Occupational Health
Public Health, Global Health, Social Medicine and Epidemiology
Obstetrics, Gynecology and Reproductive Medicine
DOI
10.1007/s43032-020-00229-z