Candida intermedia CBS 141442: A novel glucose/xylose co-fermenting isolate for lignocellulosic bioethanol production
Journal article, 2020

The present study describes the isolation of the novel strain Candida intermedia CBS 141442 and investigates the potential of this microorganism for the conversion of lignocellulosic streams. Different C. intermedia clones were isolated during an adaptive laboratory evolution experiment under the selection pressure of lignocellulosic hydrolysate and in strong competition with industrial, xylose-fermenting Saccharomyces cerevisiae cells. Isolates showed different but stable colony and cell morphologies when growing in a solid agar medium (smooth, intermediate and complex morphology) and liquid medium (unicellular, aggregates and pseudohyphal morphology). Clones of the same morphology showed similar fermentation patterns, and the C. intermedia clone I5 (CBS 141442) was selected for further testing due to its superior capacity for xylose consumption (90% of the initial xylose concentration within 72 h) and the highest ethanol yields (0.25 ± 0.02 g ethanol/g sugars consumed). Compared to the well-known yeast Scheffersomyces stipitis, the selected strain showed slightly higher tolerance to the lignocellulosic-derived inhibitors when fermenting a wheat straw hydrolysate. Furthermore, its higher glucose consumption rates (compared to S. stipitis) and its capacity for glucose and xylose co-fermentation makes C. intermedia CBS 141442 an attractive microorganism for the conversion of lignocellulosic substrates, as demonstrated in simultaneous saccharification and fermentation processes.

Yeast robustness

Biofuels

Simultaneous saccharification and fermentation

Yeast morphology

Cell factory

Author

A. D. Moreno

Centro de Investigaciones Energeticas, Medioambientales y Tecnologicas (Ciemat)

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Elia Tomas-Pejo

Industrial biotechnology

Instituto IMDEA Energía

Lisbeth Olsson

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Cecilia Geijer

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Energies

1996-1073 (ISSN)

Vol. 13 20 5363

Subject Categories

Microbiology

Biocatalysis and Enzyme Technology

Other Industrial Biotechnology

DOI

10.3390/en13205363

More information

Latest update

11/26/2020