Long-term in vivo survival of 3D-bioprinted human lipoaspirate-derived adipose tissue: proteomic signature and cellular content
Journal article, 2022

Three-dimensional (3D)-bioprinted lipoaspirate-derived adipose tissue (LAT) is a potential alternative to lipo-injection for correcting soft-tissue defects. This study investigated the long-term in vivo survival of 3D-bioprinted LAT and its proteomic signature and cellular composition. We performed proteomic and multicolour flow cytometric analyses on the lipoaspirate and 3D-bioprinted LAT constructs were transplanted into nude mice, followed by explantation after up to 150 days. LAT contained adipose-tissue-derived stem cells (ASCs), pericytes, endothelial progenitor cells (EPCs) and endothelial cells. Proteomic analysis identified 6,067 proteins, including pericyte markers, adipokines, ASC secretome proteins, proangiogenic proteins and proteins involved in adipocyte differentiation and developmental morphogenic signalling, as well as proteins not previously described in human subcutaneous fat. 3D-bioprinted LAT survived for 150 days in vivo with preservation of the construct shape and size. Furthermore, we identified human blood vessels after 30 and 150 days in vivo, indicating angiogenesis from capillaries. These results showed that LAT has a favourable proteomic signature, contains ASCs, EPCs and blood vessels that survive 3D bioprinting and can potentially facilitate angiogenesis and successful autologous fat grafting in soft-tissue reconstruction.

Lipoaspirate-derived adipose tissue

flow cytometry

3D bioprinting

endothelial progenitor cells/EPCs

adipose-derived stem cells/ASCs

proteomics

Author

Karin Säljö

University of Gothenburg

Sahlgrenska University Hospital

Peter Apelgren

University of Gothenburg

Sahlgrenska University Hospital

Linnea Strid Orrhult

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry

Susann Li

Sahlgrenska University Hospital

Matteo Amoroso

Sahlgrenska University Hospital

University of Gothenburg

Paul Gatenholm

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry

Lars Kölby

Sahlgrenska University Hospital

University of Gothenburg

Adipocyte

2162-3945 (ISSN) 2162-397X (eISSN)

Vol. 11 1 34-46

Subject Categories

Cell and Molecular Biology

Immunology in the medical area

Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

DOI

10.1080/21623945.2021.2014179

PubMed

34957918

More information

Latest update

1/10/2022