RNA Interference (RNAi ) as a Tool for High-Resolution Phenotypic Screening of the Pathogenic Yeast Candida glabrata
Book chapter, 2022

After its discovery RNA interference (RNAi) has become a powerful tool to study gene functions in different organisms. RNAi has been applied at genome-wide scale and can be nowadays performed using high-throughput automated systems (robotics). The simplest RNAi process requires the expression of two genes (Dicer and Argonaute) to function. To initiate the silencing, constructs generating either double-strand RNA or antisense RNA are required. Recently, RNAi was reconstituted by expressing Saccharomyces castellii genes in the human pathogenic yeast Candida glabrata and was used to identify new genes related to the virulence of this pathogen.In this chapter, we describe a method to make the C. glabrata pathogenic yeast competent for RNAi and to use RNA silencing as a tool for low- or high-resolution phenotypic screening in this species.

RNAi

Yeast

Functional genomics

Candida glabrata

Pathogenic yeast

Author

Andreas Tsouris

University of Strasbourg

Joseph Schacherer

University of Strasbourg

Institut Universitaire de France

Olena Ishchuk

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Methods in Molecular Biology

10643745 (ISSN) 1940-6029 (eISSN)

Vol. 2477 313-330

Subject Categories

Medical Genetics

Bioinformatics and Systems Biology

Genetics

DOI

10.1007/978-1-0716-2257-5_18

PubMed

35524125

More information

Latest update

7/12/2024