In vitro Applications of the Terpene Mini-Path 2.0
Journal article, 2022
In 2019 four groups reported independently the development of a simplified enzymatic access to the diphosphates (IPP and DMAPP) of isopentenol and dimethylallyl alcohol (IOH and DMAOH). The former are the two universal precursors of all terpenes. We report here on an improved version of what we call the terpene mini-path as well as its use in enzymatic cascades in combination with various transferases. The goal of this study is to demonstrate the in vitro utility of the TMP in, i) synthesizing various natural terpenes, ii) revealing the product selectivity of an unknown terpene synthase, or iii) generating unnatural cyclobutylated terpenes.
terpene mini-path
(+)-δ-cadinol
tryprostatin B
cyclobutyl terpenoid derivatives
farnesyl diphosphate
Author
Julie Couillaud
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Institut des Sciences Moléculaires de Marseille
Agnès Amouric
Institut des Sciences Moléculaires de Marseille
Elise Courvoisier-Dezord
Institut des Sciences Moléculaires de Marseille
Létitia Leydet
Institut des Sciences Moléculaires de Marseille
Nicolas Schweitzer
Institut des Sciences Moléculaires de Marseille
Marie Noëlle Rosso
Aix Marseille University
Hayat Hage
Aix Marseille University
Margot Loussouarn-Yvon
Aix Marseille University
Renaud Vincentelli
Aix Marseille University
Jean Louis Petit
Institut de Biologie François Jacob
Véronique de Berardinis
Institut de Biologie François Jacob
Mireille Attolini
Institut des Sciences Moléculaires de Marseille
Marc Maresca
Institut des Sciences Moléculaires de Marseille
Katia Duquesne
Institut des Sciences Moléculaires de Marseille
Gilles Iacazio
Institut des Sciences Moléculaires de Marseille
ChemBioChem
1439-4227 (ISSN) 1439-7633 (eISSN)
Vol. 23 24 e202200595Subject Categories
Biocatalysis and Enzyme Technology
Organic Chemistry
Other Industrial Biotechnology
DOI
10.1002/cbic.202200595
PubMed
36269004