Structural and functional properties of collagen isolated from lumpfish and starfish using isoelectric precipitation vs salting out
Journal article, 2023

The possibility of replacing the very time and resource demanding salting out (SO) method with isoelectric precipitation (IP) during collagen extraction from common starfish and lumpfish was investigated. The effect of IP on yield, structural and functional properties of the collagens was therefore compared with SO. Application of IP resulted in a higher or similar collagen mass yield compared with SO from starfish and lumpfish, respectively. However, the purity of collagens recovered with IP was lower than those recovered with SO. Replacing SO with IP did not affect polypeptide pattern and tropohelical structural integrity of collagen from the two resources as revealed with SDS-PAGE and FTIR analysis. Thermal stability and fibril formation capacity of collagens recovered with IP were also well preserved. Overall, the results showed that the IP can be a promising resource smart alternative for the classic SO precipitation during collagen extraction from marine resources.

Isoelectric precipitation

Lumpfish

Fibril formation

Starfish

Marine collagen

Author

Naveen Kumar Vate

Centurion University of Technology and Management

Chalmers, Life Sciences, Food and Nutrition Science

Przemyslaw Pawel Strachowski

Chalmers, Life Sciences, Food and Nutrition Science

Ingrid Undeland

Chalmers, Life Sciences, Food and Nutrition Science

Mehdi Abdollahi

Chalmers, Life Sciences, Food and Nutrition Science

Food Chemistry: X

25901575 (eISSN)

Vol. 18 100646

Kommersiell utvinning av kollagen och kitin från underutnyttjade marina arter (BlueCC )

Formas (2019-02362), 2019-12-10 -- 2023-09-30.

Blue Biorefining: a holistic solution for increasing resource efficiency in seafood industry (BlueBio)

VINNOVA (2021-03724), 2021-12-01 -- 2024-12-01.

Subject Categories

Polymer Chemistry

Bio Materials

Chemical Process Engineering

DOI

10.1016/j.fochx.2023.100646

PubMed

37008722

More information

Latest update

4/14/2023