Novel ruthenium-doped vanadium carbide/polymeric nanohybrid sensor for acetaminophen drug detection in human blood
Journal article, 2023

The use of advanced electroactive catalysts enhances the performance of electrochemical biosensors in real-time biomonitoring and has received much attention owing to its excellent physicochemical and electrochemical possessions. In this work, a novel biosensor was developed based on the electrocatalytic activity of functionalized vanadium carbide (VC) material, including VC@ruthenium (Ru), VC@Ru-polyaniline nanoparticles (VC@Ru-PANI-NPs) as non-enzymatic nanocarriers for the fabrication of modified screen-printed electrode (SPE) to detect acetaminophen in human blood. As-prepared materials were characterized using SEM, TEM, XRD, and XPS techniques. Biosensing was carried out using cyclic voltammetry and differential pulse voltammetry techniques and has revealed imperative electrocatalytic activity. A quasi-reversible redox method of the over-potential of acetaminophen increased considerably compared with that at the modified electrode and the bare SPE. The excellent electrocatalytic behaviour of VC@Ru-PANI-NPs/SPE is attributed to its distinctive chemical and physical properties, including rapid electron transfer, striking ᴫ-ᴫ interface, and strong adsorptive capability. This electrochemical biosensor exhibits a detection limit of 0.024 μM, in a linear range of 0.1–382.72 μM with a reproducibility of 2.45 % relative standard deviation, and a good recovery from 96.69 % to 105.59 %, the acquired results ensure a better performance compared with previous reports. The enriched electrocatalytic activity of this developed biosensor is mainly credited to its high surface area, better electrical conductivity, synergistic effect, and abundant electroactive sites. The real-world utility of the VC@Ru-PANI-NPs/SPE-based sensor was ensured via the investigation of biomonitoring of acetaminophen in human blood samples with satisfactory recoveries.

Electrochemical sensor

Conductive polymer

Two-dimensional metal carbide

Human plasma

Acetaminophen

Author

Murugesan Duraisamy

Kaohsiung Medical University

University of Science, Malaysia

SSN College of Engineering

Mari Elancheziyan

Kaohsiung Medical University

Muthusankar Eswaran

Chalmers, Life Sciences, Systems and Synthetic Biology

Kaohsiung Medical University

Sivarasan Ganesan

Kaohsiung Medical University

Anees A. Ansari

King Saud University College of Science

Govindaraj Rajamanickam

SSN College of Engineering

Siew Ling Lee

Universiti Teknologi Malaysia

Pei Chien Tsai

Kaohsiung Medical University

Saveetha School of Engineering

Yi Hsun Chen

Kaohsiung Medical University

Vinoth Kumar Ponnusamy

Kaohsiung Medical University

National Sun Yat-Sen University

International Journal of Biological Macromolecules

0141-8130 (ISSN) 18790003 (eISSN)

Vol. 244 125329

Subject Categories

Analytical Chemistry

Pharmacology and Toxicology

DOI

10.1016/j.ijbiomac.2023.125329

PubMed

37307970

More information

Latest update

6/29/2023