Adipose-Derived Stromal Cells Preserve Pancreatic Islet Function in a Transplantable 3D Bioprinted Scaffold
Journal article, 2023

Intra-portal islet transplantation is currently the only clinically approved beta cell replacement therapy, but its outcome is hindered by limited cell survival due to a multifactorial reaction against the allogeneic tissue in liver. Adipose-derived stromal cells (ASCs) can potentially improve the islet micro-environment by their immunomodulatory action. The challenge is to combine both islets and ASCs in a relatively easy and consistent long-term manner in a deliverable scaffold. Manufacturing the 3D bioprinted double-layered scaffolds with primary islets and ASCs using a mix of alginate/nanofibrillated cellulose (NFC) bioink is reported. The diffusion properties of the bioink and the supportive effect of human ASCs on islet viability, glucose sensing, insulin secretion, and reducing the secretion of pro-inflammatory cytokines are demonstrated. Diabetic mice transplanted with islet-ASC scaffolds reach normoglycemia seven days post-transplantation with no significant difference between this group and the group received islets under the kidney capsules. In addition, animals transplanted with islet-ASC scaffolds stay normoglycemic and show elevated levels of C-peptide compared to mice transplanted with islet-only scaffolds. The data present a functional 3D bioprinted scaffold for islets and ASCs transplanted to the extrahepatic site and suggest a possible role of ASCs on improving the islet micro-environment.

ASC

3D bioprinting

in vivo

islets

scaffolds

nanocellulose

alginate

Author

Shadab Abadpour

University of Oslo

Oslo University Hospital

Institute for Surgical Research

Essi M. Niemi

University of Oslo

Institute for Surgical Research

Aker University Hospital

Linnea Strid Orrhult

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry

Carolin Hermanns

Maastricht University

Rick de Vries

Maastricht University

Liebert Parreiras Nogueira

University of Oslo

Havard J. Haugen

University of Oslo

Dag Josefsen

Rikshospitalet-Radiumhospitalet HF

Stefan Krauss

Oslo University Hospital

University of Oslo

Paul Gatenholm

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry

Cellheal AS

Aart van Apeldoorn

Maastricht University

Hanne Scholz

Institute for Surgical Research

Rikshospitalet-Radiumhospitalet HF

University of Oslo

Oslo University Hospital

Advanced healthcare materials

2192-2640 (ISSN) 2192-2659 (eISSN)

Vol. 12 32 2300640

Subject Categories

Surgery

Cell and Molecular Biology

Gastroenterology and Hepatology

DOI

10.1002/adhm.202300640

PubMed

37781993

More information

Latest update

3/7/2024 9