Structure and interfacial properties of phospholipid-containing sponge nanoparticles and their interaction with myoglobin
Journal article, 2025
Hypothesis: Sponge phase (L3) lipid nanoparticles (L3-NPs) have been shown to have large potential for the encapsulation of biomolecules, such as enzymes, with applications in food and pharmaceutical science. In this study, we introduce new formulations of L3-NPs including the phospholipids dioleoylphosphatidylcholine (DOPC) and dioleoyltrimethylammonium propane (DOTAP). The interaction of these new L3-NPs with myoglobin is of interest for the development of iron supplements which can be incorporated during food processing. Experiments: We characterized the sample structure by small-angle X-ray scattering (SAXS) measurements with and without the addition of myoglobin. We also tested the myoglobin-lipid interaction in an experimental setup that mimicked the interface between the bilayer and water channels within the bicontinuous sponge structure. This included spreading the L3-NPs onto a hydrophilic surface to form supported lipid bilayers and characterizing their interaction with myoglobin by means of quartz crystal microbalance with dissipation monitoring and polarized neutron reflectometry. Findings: SAXS data indicate that the new formulations containing DOPC and DOTAP formed a sponge phase in the bulk. The data from the surface techniques showed that deposited bilayers containing DOPC were largely unaffected by the addition of myoglobin, whereas those without DOPC were destabilized and partially removed.
Polarised neutron reflectometry
Supported lipid bilayers
Sponge-phase nanoparticles
Author
Alessandra Luchini
University of Perugia
Marshall R. Machingauta
Lund University
Sebastian Köhler
Lund Institute of Advanced Neutron and X-ray Science (LINXS)
Lund University
Jennifer Gilbert
Chalmers, Life Sciences, Chemical Biology
Lund University
Ivan P. Yakimenko
Linköping University
Jens Birch
Linköping University
K. Järrendahl
Linköping University
Joshaniel F.K. Cooper
European Spallation Source (ESS)
Sjoerd Stendahl
Uppsala University
Linköping University
Sean Langridge
STFC Rutherford Appleton Laboratory
Christy Kinane
STFC Rutherford Appleton Laboratory
Andrew J. Caruana
STFC Rutherford Appleton Laboratory
Olga Dikaia
Koenigssystems UG
Aleksandr Goikhman
Koenigssystems UG
Alexei Vorobiev
Institut Laue-Langevin
Uppsala University
Anton Devishvili
Institut Laue-Langevin
Björgvin Hjörvarsson
Uppsala University
T. Nylander
Lund University
Sungkyunkwan University
Lund Institute of Advanced Neutron and X-ray Science (LINXS)
Journal of Colloid and Interface Science
0021-9797 (ISSN) 1095-7103 (eISSN)
Vol. 697 137879Subject Categories (SSIF 2025)
Molecular Biology
Physical Chemistry
DOI
10.1016/j.jcis.2025.137879
PubMed
40424802