Direct immobilization of cholesteryl-TEG-modified oligonucleotides onto hydrophobic SU-8 surfaces
Journal article, 2007

We introduce a rapid, simple one-step procedure for the high-yield immobilization of cholesteryl- tetraethyleneglycol-modified oligonucleotides ( chol- DNA) at hydrophobic sites made of SU-8 photoresist. Topographic structures of SU-8 were microfabricated on microscope glass coverslips sputtered with a Ti/Au layer. Upon application, chol-DNA adsorbed to the SU-8 structures from solution, leaving the surrounding gold surface free of chol- DNA. chol-DNA immobilization is complete within 15 min and yields a surface coverage in the range of 20- 95 pmol/ cm(2), which corresponds to a film density of 10(12)- 10(13) molecules/cm(2). chol-DNA immobilization is stable and can be sustained despite rinsing, drying, dry storage for several hours, and rehydration of chips. Furthermore, complementary DNA in solution hybridizes efficiently to immobilized chol-DNA. We introduce a rapid, simple one-step procedure for the high-yield immobilization of cholesteryl-tetraethyleneglycol-modified oligonucleotides (chol-DNA) at hydrophobic sites made of SU-8 photoresist. Topographic structures of SU-8 were microfabricated on microscope glass coverslips sputtered with a Ti/Au layer. Upon application, chol-DNA adsorbed to the SU-8 structures from solution, leaving the surrounding gold surface free of chol-DNA. chol-DNA immobilization is complete within 15 min and yields a surface coverage in the range of 20-95 pmol/cm(2), which corresponds to a film density of 10(12)-10(13) molecules/cm(2). chol-DNA immobilization is stable and can be sustained despite rinsing, drying, dry storage for several hours, and rehydration of chips. Furthermore, complementary DNA in solution hybridizes efficiently to immobilized chol-DNA.

Author

Yavuz Erkan

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Ilja Czolkos

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Aldo Jesorka

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Marcus Wilhelmsson

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Owe Orwar

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Langmuir

07437463 (ISSN) 15205827 (eISSN)

Vol. 23 10 5259-5263

Subject Categories

Chemical Sciences

DOI

10.1021/la7005502

More information

Created

10/8/2017