Precipitation of calcium phosphate in the presence of albumin on titanium implants with four different possibly bioactive surface preparations. An in vitro study
Journal article, 2008
The aim of the present study was to compare the
nucleating behaviour on four types of bioactive surfaces by
using the simulated body fluid (SBF) model with the presence
albumin. Titanium discs were blasted (B) and then
prepared by alkali and heat treatment (AH), anodic oxidation
(AO), fluoridation (F), or hydroxyapatite coating (HA).
The discs were immersed in SBF with 4.5 mg/ml albumin
for 3 days, 1, 2, 3 and 4 weeks and analysed with scanning
electron microscopy/energy dispersive X-ray analysis
(SEM/EDX) and X-ray photoelectron spectroscopy (XPS).
Topographic surface characterisation was performed with a
contact stylus profilometer. The results demonstrated that
the bioactive surfaces initiated an enhanced calcium phosphate
(CaP) formation and a more rapid increase of protein
content was present on the bioactive surfaces compared to
the blasted control surface. The observation was present on
all bioactive surfaces. The fact that there was a difference
between the bioactive surfaces and the blasted control surface
with respect to precipitation of CaP and protein content
on the surfaces support the fact that there may be biochemical
advantages in vivo by using a bioactive surface.