Optimization study of citrus wastes saccharification by dilute-acid hydrolysis
Journal article, 2008

The effects of time, acid concentration, temperature and solid concentration on dilute-acid hydrolysis of orange peels were investigated. A central composite rotatable experimental design (CCRD) was applied to study the individual effects of these hydrolysis factors and also their interdependence effects. The enzymatic hydrolysis of the peels by cellulase, β-glucosidase, and pectinase enzymes resulted in 72% dissolution of the peels, including 18.7% galacturonic acid and 53.3% of a total of glucose, fructose, galactose, and arabinose. Dilute-acid hydrolysis up to 210°C was not able to hydrolyze pectin to galacturonic acid. However, the sugar polymers were hydrolyzed at relatively low temperature. The optimum results were obtained at 116°C, 0.5% sulfuric acid concentration, 6% solid fraction, and 12.9 min retention time. Under these conditions, the total sugars obtained at 41.8% dry peels and 2.6% of total hexose sugars were further degraded to hydroxymethylfurfural (HMF). No furfural was detected through these experiments from decomposition of pentoses.

Sugar optimization

Dilute-acid hydrolysis

Experimental design

Orange peel


Farid Talebnia Rowshan

Chalmers, Chemical and Biological Engineering, Chemical Reaction Engineering

Mohammad Pour Bafrani

Chalmers, Chemical and Biological Engineering, Chemical Reaction Engineering

M. Lundin

University of Borås

Mohammad Taherzadeh Esfahani

University of Borås


1930-2126 (ISSN) 19302126 (eISSN)

Vol. 3 1 108-122

Subject Categories

Organic Chemistry

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3/8/2018 9