Oxygen radical production and severity of the Guillain--Barré syndrome.
Journal article, 2007

The NADPH oxidase-dependent formation of reactive oxygen species ("oxygen radicals") by phagocytic cells constitutes an important part of the innate immune defence against microorganisms. Recent studies in animal models imply that a deficient function of the NADPH oxidase may be linked to the development of autoimmunity, but a link between oxygen radical production and severity of autoimmune disease in humans has not been established. We have examined the oxygen radical production in peripheral blood leukocytes from patients with the Guillain-Barré syndrome (GBS). Leukocytes from GBS patients in a stationary phase 1-5 years after their acute episode were activated by the formyl peptide receptor (FPR) ligand formyl-Met-Leu-Phe (fMLF) or the closely related formyl peptide like receptor 1 (FPRL1) ligand Trp-Lys-Tyr-Met-Val-Met-NH2 (WKYMVM). The patients were dichotomized according to severity by 1) the requirement of intensive care unit treatment and 2) the ability to walk independently after 3 months. Our data show that the amount of superoxide release following challenge with either of the two agonists fMLF and WKYMVM was significantly lower in patients requiring intensive care unit treatment or unable to walk after 3 months. Results obtained with the global activator phorbol myristate acetate, as well as with fMLF in TNF alpha-primed leukocytes, suggested that the deficiency of oxygen radical production in patients with severe GBS was the result of a specific deficiency of radical production in response to FPR/FPRL1 ligands rather than an inherent deficiency of NADPH oxidase function.

Severity

Leukocytes

NADPH Oxidase

Reactive Oxygen Species

Guillain-Barre Syndrome

Author

Natalia Mossberg

University of Gothenburg

Oluf Andersen

University of Gothenburg

Staffan Nilsson

Chalmers, Mathematical Sciences

University of Gothenburg

Claes Dahlgren

University of Gothenburg

Kristoffer Hellstrand

University of Gothenburg

Magnus Lindh

University of Gothenburg

Åke Svedhem

University of Gothenburg

Tomas Bergström

University of Gothenburg

Charlotta Movitz

University of Gothenburg

Journal of Neuroimmunology

0165-5728 (ISSN)

Vol. 192 1-2 186-91

Subject Categories

Microbiology in the medical area

DOI

10.1016/j.jneuroim.2007.09.020

PubMed

17945354

More information

Created

10/7/2017