Chemical imaging of glucose by CARS microscopy
Journal article, 2010
Glucose is one of the most fundamental molecules within life and bioengineering sciences. Present understanding of its role in cellular and bioengineering processes relies primarily on invasive, large-scale biochemical analysis, providing no spatial information on glucose pools or fluxes. This work identifies an emerging microscopy technique based on coherent anti-Stokes Raman scattering (CARS), which fulfills the need of quantitative imaging of glucose at the single-cell level with submicrometer resolution. No sample preparation with reporter molecules is required, ensuring that the low-weight metabolite is studied under natural conditions. The potential of CARS microscopy is illustrated by quantitatively mapping glucose fluxes and distributions in a microfluidic bioreactor and in lipid-bilayer vesicles, the latter as a model for glucose transmembrane transport. Furthermore, the metabolic response to a glucose pulse was monitored in living yeast cells. This study signifies a new era within CARS microscopy for its use of monitoring carbohydrates, in particular glucose which is one of the most abundant molecules in nature.
liposome
visualization
raman scattering microscopy
stress
yeast
coherent anti-Stokes Raman scattering (CARS)
CARS microscopy
spectroscopy
insulin-resistance
microspectroscopy
carbohydrates
transporters
saccharomyces-cerevisiae
cells
glucose
yeast
Author
Madeleine Åkeson
Chalmers, Chemical and Biological Engineering, Molecular Imaging
Christian Brackmann
Chalmers, Chemical and Biological Engineering, Molecular Imaging
Lena Gustafsson
Chalmers, Chemical and Biological Engineering, Molecular Imaging
Annika Enejder
Chalmers, Chemical and Biological Engineering, Molecular Imaging
Journal of Raman Spectroscopy
0377-0486 (ISSN) 1097-4555 (eISSN)
Vol. 41 12 1348-1354Subject Categories
Chemical Sciences
DOI
10.1002/jrs.2619