Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light
Journal article, 2011

Bicelles are excellent membrane-mimicking hosts for a dynamic and structural study of solutes with NMR, but the magnetic fields required for their alignment are hard to apply to optical conditions. Here we demonstrate that bicellar mixtures can be aligned by shear forces in a Couette flow cell, to provide orientation of membrane-bound retinoic acid, pyrene and cytochrome c (cyt c) protein, conveniently studied with linear dichroism spectroscopy.

nmr

proteins

model

linear dichroism spectroscopy

binding

micelles

Author

Maxim Kogan

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Tamas Beke-Somfai

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Bengt Nordén

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Chemical Communications

1359-7345 (ISSN)

Vol. 47 26 7356-7358

Supramolecular Motive Power (SUMO)

European Commission (FP7), 2009-01-01 -- 2014-12-31.

Areas of Advance

Energy

Life Science Engineering (2010-2018)

Materials Science

Subject Categories

Chemical Sciences

DOI

10.1039/c1cc12313k

More information

Created

10/6/2017