CARS microscopy for the visualization of micrometer-sized iron oxide MRI contrast agents in living cells
Journal article, 2011

Micrometer-sized iron oxide particles (MPIOs) attract increasing interest as contrast agents for cellular tracking by clinical Magnetic Resonance Imaging (MRI). Despite the great potential of MPIOs for in vivo imaging of labeled cells, little is known on the intracellular localization of these particles following uptake due to the lack of techniques with the ability to monitor the particle uptake in vivo at single-cell level. Here, we show that coherent anti-Stokes Raman scattering (CARS) microscopy enables non-invasive, label-free imaging of MPIOs in living cells with sub-micron resolution in three dimensions. CARS allows simultaneous visualization of the cell framework and the MPIOs, where the particles can be readily distinguished from other cellular components of comparable dimensions, and localized inside the cell.

in-vivo

liver repopulation

single cells

cellular mri

mri

dendritic cells

2-photon absorption

human hepatocyte transplantation

metabolic-disorders

raman scattering microscopy

stem-cells

Author

G. Rago

FOM Institute for Atomic and Molecular Physics - AMOLF

C. M. Langer

Charité University Medicine Berlin

Christian Brackmann

Chalmers, Chemical and Biological Engineering, Molecular Imaging

J. P. R. Day

FOM Institute for Atomic and Molecular Physics - AMOLF

K. F. Domke

FOM Institute for Atomic and Molecular Physics - AMOLF

N. Raschzok

Charité University Medicine Berlin

C. Schmidt

Microparticles GmbH

I. M. Sauer

Charité University Medicine Berlin

Annika Enejder

Chalmers, Chemical and Biological Engineering, Molecular Imaging

M. T. Mogl

Charité University Medicine Berlin

M. Bonn

FOM Institute for Atomic and Molecular Physics - AMOLF

Biomedical Optics Express

2156-7085 (ISSN)

Vol. 2 9 2470-2483

Subject Categories

Chemical Sciences

DOI

10.1364/BOE.2.002470

More information

Latest update

3/5/2018 8