Osteogenesis-inducing calcium phosphate nanoparticle precursors applied to titanium surfaces
Journal article, 2013

This study investigated the effects of the morphology and physicochemical properties of calcium phosphate (CaP) nanoparticles on osteogenesis. Two types of CaP nanoparticles were compared, namely amorphous calcium phosphate (ACP) nano-spheres (diameter: 9-13 nm) and poorly crystalline apatite (PCA) nano-needles (30-50 nm x 2-4 nm) that closely resemble bone apatite. CaP particles were spin-coated onto titanium discs and implants; they were evaluated in cultured mouse calvarial osteoblasts, as well as after implantation in rabbit femurs. A significant dependence of CaP coatings was observed in osteoblast-related gene expression (Runx2, Colla1 and Spp1). Specifically, the PCA group presented an up-regulation of the osteospecific genes, while the ACP group suppressed the Runx2 and Colla1 expression when compared to blank titanium substrates. Both the ACP and PCA groups presented a more than three-fold increase of calcium deposition, as suggested by Alizarin red staining. The removal torque results implied a slight tendency in favour of the PCA group. Different forms of CaP nanostructures presented different biologic differences; the obtained information can be used to optimize surface coatings on biomaterials.


coated implants

osteoblast adhesion


octacalcium phosphate


ca-deficient hydroxyapatite





Wenxiao He

Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry

Martin Andersson

Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry

P. P. C. de Souza

São Paulo State University (UNESP)

C. A. D. Costa

São Paulo State University (UNESP)

E. M. Munoz

São Paulo State University (UNESP)

Humberto Osvaldo Schwartz-Filho

São Paulo State University (UNESP)

Mariko Hayashi

Malmö university

A. Hemdal

Malmö university

A. Fredel

Malmö university

Ann Wennerberg

Malmö university

Ryo Jimbo

Malmö university

Biomedical Materials (Bristol)

1748-6041 (ISSN) 1748-605X (eISSN)

Vol. 8 3 035007

Subject Categories

Materials Engineering

Chemical Sciences



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