Electrospun nanofibrous cellulose scaffolds with controlled microarchitecture
Journal article, 2014

Introducing porosity in electrospun scaffolds is critical to improve cell penetration and nutrient diffusion for tissue engineering. Nanofibrous cellulose scaffolds were prepared by electrospinning cellulose acetate (CA) followed by saponification to regenerate cellulose. Using a computer-assisted design approach, scaffolds underwent laser ablation resulting in pores with diameters between 50 and 300 mu m. without damaging or modifying the surrounding scaffold area. A new mineralization method was employed in conjunction with microablation using commercial phosphate buffered saline (PBS) to soak carboxymethylcellulose surface-modified electrospun scaffolds. The resulting crystals within the scaffold on the interior of the pore had a calcium to phosphate ratio of 1.56, similar to hydroxyapatite. It was observed that porosity of the cellulose scaffolds enhanced osteoblast cell attachment at the edge of the pores, while mineralization enhanced overall cell density.

Scaffold

Porosity

Bone regeneration

Laser

Hydroxyapatite

Cellulose

Author

K. Rodriguez

Virginia Polytechnic Institute and State University

Johan Sundberg

Chalmers, Chemical and Biological Engineering, Polymer Technology

Paul Gatenholm

Chalmers, Chemical and Biological Engineering, Polymer Technology

Wallenberg Wood Science Center (WWSC)

S. Renneckar

Virginia Polytechnic Institute and State University

Carbohydrate Polymers

0144-8617 (ISSN)

Vol. 100 143-149

Subject Categories

Polymer Technologies

DOI

10.1016/j.carbpol.2012.12.037

More information

Latest update

8/27/2018