In situ forming spruce xylan-based hydrogel for cell immobilization
Journal article, 2014

An in situ forming spruce xylan-based hydrogel was synthesized in two steps with the intended use of cell encapsulation and in vivo delivery. First, bioconjugate was obtained through the reaction of glucuronic acid groups from xylan backbone with tyramine (TA). After that, the gelation process was enabled by enzymatic crosslinking of the phenol-containing TA-xylan conjugate. Exhibiting an exponential increase in the storage modulus, a 3D gel network was formed in about 20s. The designed gel showed extensive swelling and retained its mechanical integrity for more than two months. Mesenchymal stem cells were encapsulated in the hydrogel and cultured for one week. The cells retained their adipogenic differentiation capacity inside the gel, as verified by lipid accumulation. From these facts, we conclude that spruce xylan is a promising precursor for in situ forming hydrogels and should be evaluated further for tissue engineering purposes.

Author

Volodymyr Kuzmenko

Chalmers, Applied Physics, Electronics Material and Systems Laboratory

Daniel Hägg

Chalmers, Chemical and Biological Engineering, Polymer Technology

Guillermo Toriz Gonzalez

Chalmers, Chemical and Biological Engineering, Polymer Technology

Paul Gatenholm

Wallenberg Wood Science Center (WWSC)

Chalmers, Chemical and Biological Engineering, Polymer Technology

Carbohydrate Polymers

0144-8617 (ISSN)

Vol. 102 1 862-868

Subject Categories

Polymer Chemistry

DOI

10.1016/j.carbpol.2013.10.077

More information

Latest update

8/24/2018