Biofilm formation on nanostructured hydroxyapatite-coated titanium
Journal article, 2014

Biofilm formation on medical devices is a common cause of implant failure, especially regarding implants that breach the epithelial tissue, so-called transcutaneous implants. Nanotechnology and the development of new nanomaterials have given the opportunity to design nanotextured implant surfaces. Such surfaces have been studied using various in vitro methods showing that nanosized features strongly benefit bone cell growth. However, little is known on how nanostructured features affect biofilm formation. The aim of this study was therefore to examine the shape- and chemical-dependent effect of a nanostructured hydroxyapatite (HA) coating on the degree of Staphylococcus epidermidis biofilm formation. Three different types of nanosized HA particles having different shapes and calcium to phosphate ratios were compared to uncoated turned titanium using safranin stain in a biofilm assay and confocal laser scanning microscopy (CLSM) for assessment of biofilm biomass and bacterial volume, respectively. No difference in biofilm biomass was detected for the various surfaces after 6 h incubation with S. epidermidis. Additionally, image analysis of CLSM Z-stacks confirmed the biofilm assay and showed similar results. In conclusion, the difference in nanomorphology and chemical composition of the surface coatings did not influence the adhesion and biofilm formation of S. epidermidis.

Staphylococcus epidermidis






Emma Westas

Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry

M. Gillstedt

Sahlgrenska University Hospital

J. Lönn-Stensrud

University of Oslo

E.M. Bruzell

Nordisk Institutt for Odontologiske Materialer

Martin Andersson

Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry

Journal of Biomedical Materials Research - Part A

1549-3296 (ISSN)

Vol. 102 4 1063-1070

Driving Forces

Sustainable development

Innovation and entrepreneurship

Subject Categories


Biological Sciences


Nano Technology


Medical Materials

Areas of Advance

Materials Science



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5/8/2018 6