2-Butanol and Butanone Production in Saccharomyces cerevisiae through Combination of a B-12 Dependent Dehydratase and a Secondary Alcohol Dehydrogenase Using a TEV-Based Expression System
Journal article, 2014
2-Butanol and its chemical precursor butanone (methyl ethyl ketone -MEK) are chemicals with potential uses as biofuels and biocommodity chemicals. In order to produce 2-butanol, we have demonstrated the utility of using a TEV-protease based expression system to achieve equimolar expression of the individual subunits of the two protein complexes involved in the B-12-dependent dehydratase step (from the pdu-operon of Lactobacillus reuterii), which catalyze the conversion of meso-2,3-butanediol to butanone. We have furthermore identified a NADH dependent secondary alcohol dehydrogenase (Sadh from Gordonia sp.) able to catalyze the subsequent conversion of butanone to 2-butanol. A final concentration of 4 +/- 0.2 mg/L 2-butanol and 2 +/- 0.1 mg/L of butanone was found. A key factor for the production of 2-butanol was the availability of NADH, which was achieved by growing cells lacking the GPD1 and GPD2 isogenes under anaerobic conditions.