Normalization of Host Intestinal Mucus Layers Requires Long-Term Microbial Colonization
Journal article, 2015

The intestinal mucus layer provides a barrier limiting bacterial contact with the underlying epithelium. Mucus structure is shaped by intestinal location and the microbiota. To understand how commensals modulate gut mucus, we examined mucus properties under germ-free (GF) conditions and during microbial colonization. Although the colon mucus organization of GF mice was similar to that of conventionally raised (Convr) mice, the GF inner mucus layer was penetrable to bacteria-sized beads. During colonization, in which GF mice were gavaged with Convr microbiota, the small intestine mucus required 5 weeks to be normally detached and colonic inner mucus 6 weeks to become impenetrable. The composition of the small intestinal microbiota during colonization was similar to Convr donors until 3 weeks, when Bacteroides increased, Firmicutes decreased, and segmented filamentous bacteria became undetectable. These findings highlight the dynamics of mucus layer development and indicate that studies of mature microbe-mucus interactions should be conducted weeks after colonization.

model

secretion

mucin

immune

homeostasis

bacteria

glycosylation

gut microbiota

mouse stomach

ecosystem

functional cftr channel

Author

Malin E V Johansson

University of Gothenburg

Hedvig E Jakobsson

University of Gothenburg

Jessica Holmén Larsson

University of Gothenburg

André Schütte

University of Gothenburg

Anna Ermund

University of Gothenburg

Ana María Rodríguez-Piñeiro

University of Gothenburg

Liisa Arike

University of Gothenburg

Catharina Wising

University of Gothenburg

Frida Svensson

University of Gothenburg

Fredrik Bäckhed

University of Gothenburg

Gunnar C. Hansson

University of Gothenburg

Cell Host and Microbe

1931-3128 (ISSN)

Vol. 18 5 582-592

Subject Categories

Microbiology

DOI

10.1016/j.chom.2015.10.007

More information

Created

10/10/2017