Determining Number Concentrations and Diameters of Polystyrene Particles by Measuring the Effective Refractive Index of Colloids Using Surface Plasmon Resonance
Journal article, 2016

The capabilities of surface plasmon resonance (SPR) for characterization of colloidal particles were evaluated for 100, 300, and 460 nm nominal diameter polystyrene (PS) latexes. First the accuracy of measuring the effective refractive index (n(eff)) of turbid colloids using SPR was quantified. It was concluded that for submicrometer sized PS particles the accuracy is limited by the reproducibility between replicate injections of samples. An SPR method was developed for obtaining the particle mean diameter (d(part)) and the particle number concentration (c(p)) by fitting the measured n(eff) of polystyrene (PS) colloids diluted in series with theoretical values calculated using the coherent scattering theory (CST). The d(part) and c(p) determined using SPR agreed with reference values obtained from size distributions measured by scanning electron microscopy (SEM), and the mass concentrations stated by the manufacturer. The 100 nm particles adsorbed on the sensing surface, which hampered the analysis. Once the adsorption problem has been overcome, the developed SPR method has potential to become a versatile tool for characterization of colloidal particles. In particular, SPR could form the basis of rapid and accurate methods for measuring the c(p) of submicrometer particles in dispersion.

SCATTERING

DEPENDENT

OPTICAL-PROPERTIES

SYSTEM

SIZE

LIGHT-SCATTERING

TOOL

RADIATIVE-TRANSFER

NANOPARTICLE TRACKING ANALYSIS

SUSPENSION

Author

Jani Tuoriniemi

University of Gothenburg

Beatriz Moreira

University of Gothenburg

Gulnara Safina

University of Gothenburg

Chalmers, Physics, Biological Physics

Langmuir

07437463 (ISSN) 15205827 (eISSN)

Vol. 32 41 10632-10640

Subject Categories

Physical Chemistry

DOI

10.1021/acs.langmuir.6b02684

More information

Created

10/8/2017