Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink
Journal article, 2017

Cartilage lesions can progress into secondary osteoarthritis and cause severe clinical problems in numerous patients. As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes. Two bioinks were investigated: NFC with alginate (NFC/A) or hyaluronic acid (NFC/HA). Low proliferation and phenotypic changes away from pluripotency were seen in the case of NFC/HA. However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs. Moreover, a marked increase in cell number within the cartilaginous tissue was detected by 2-photon fluorescence microscopy, indicating the importance of high cell densities in the pursuit of achieving good survival after printing. We conclude that NFC/A bioink is suitable for bioprinting iPSCs to support cartilage production in co-cultures with irradiated chondrocytes.

Bone

Human

Chondrogenic Differentiation

Hydrogel

Knee

Autologous Chondrocyte Implantation

System

Self-Renewal

Articular Chondrocytes

Pluripotent Stem-Cells

Author

Duy Nguyen

Chalmers, Chemistry and Chemical Engineering, Chemical Technology, Chemical Environmental Science

Daniel Hägg

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Polymer Technology

Alma Forsman

University of Gothenburg

Josefine Ekholm

University of Gothenburg

P. Nimkingratana

Sahlgrenska Academy

Camilla Brantsing

University of Gothenburg

Theodoros Kalogeropoulos

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Polymer Technology

Samantha Zaunz

Chalmers, Chemistry and Chemical Engineering

Sebastian Concaro

University of Gothenburg

Mats Brittberg

University of Gothenburg

Anders Lindahl

University of Gothenburg

Paul Gatenholm

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Polymer Technology

Annika Enejder

Chalmers, Biology and Biological Engineering, Chemical Biology

Stina Simonsson

University of Gothenburg

Scientific Reports

2045-2322 (ISSN)

Vol. 7 1 00690

Subject Categories

Biomaterials Science

DOI

10.1038/s41598-017-00690-y

More information

Latest update

3/23/2018