Quantification of the Intracellular Life Time of Water Molecules to Measure Transport Rates of Human Aquaglyceroporins.
Journal article, 2017

Orthodox aquaporins are transmembrane channel proteins that facilitate rapid diffusion of water, while aquaglyceroporins facilitate the diffusion of small uncharged molecules such as glycerol and arsenic trioxide. Aquaglyceroporins play important roles in human physiology, in particular for glycerol metabolism and arsenic detoxification. We have developed a unique system applying the strain of the yeast Pichia pastoris, where the endogenous aquaporins/aquaglyceroporins have been removed and human aquaglyceroporins AQP3, AQP7, and AQP9 are recombinantly expressed enabling comparative permeability measurements between the expressed proteins. Using a newly established Nuclear Magnetic Resonance approach based on measurement of the intracellular life time of water, we propose that human aquaglyceroporins are poor facilitators of water and that the water transport efficiency is similar to that of passive diffusion across native cell membranes. This is distinctly different from glycerol and arsenic trioxide, where high glycerol transport efficiency was recorded.


Madelene Palmgren

University of Gothenburg

Malin Hernebring

Lund University

Stefanie Eriksson

Lund University

Karin Elbing

Lund University

Cecilia Geijer

University of Gothenburg

Samo Lasič

CR Development AB

Peter Dahl

University of Gothenburg

Jesper S Hansen

Lund University

Daniel Topgaard

Lund University

Karin Lindkvist-Petersson

University of Gothenburg

Lund University

Journal of Membrane Biology

0022-2631 (ISSN) 1432-1424 (eISSN)

Vol. 250 6 629-639

Subject Categories

Biochemistry and Molecular Biology

Areas of Advance

Life Science Engineering (2010-2018)





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7/8/2019 1