Macromolecular nanocrystal structural analysis with electron and X-rays: A comparative review
Review article, 2019

Crystallography has long been the unrivaled method that can provide the atomistic structural models of macromolecules, using either X-rays or electrons as probes. The methodology has gone through several revolutionary periods, driven by the development of new sources, detectors, and other instrumentation. Novel sources of both X-ray and electrons are constantly emerging. The increase in brightness of these sources, complemented by the advanced detection techniques, has relaxed the traditionally strict need for large, high quality, crystals. Recent reports suggest high-quality diffraction datasets from crystals as small as a few hundreds of nanometers can be routinely obtained. This has resulted in the genesis of a new field of macromolecular nanocrystal crystallography. Here we will make a brief comparative review of this growing field focusing on the use of X-rays and electrons sources.


X-ray free-electron laser

Electron diffraction


Krishna P. Khakurel

Czech Academy of Sciences

Borislav Angelov

Czech Academy of Sciences

Jakob Andreasson

Chalmers, Physics, Materials Physics

Czech Academy of Sciences


1420-3049 (ISSN)

Vol. 24 19 3490

Subject Categories

Accelerator Physics and Instrumentation

Inorganic Chemistry

Astronomy, Astrophysics and Cosmology

Areas of Advance

Materials Science





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