Microbiota-directed fibre activates both targeted and secondary metabolic shifts in the distal gut
Journal article, 2020
Beneficial modulation of the gut microbiome has high-impact implications not only in humans, but also in livestock that sustain our current societal needs. In this context, we have tailored an acetylated galactoglucomannan (AcGGM) fibre to match unique enzymatic capabilities of Roseburia and Faecalibacterium species, both renowned butyrate-producing gut commensals. Here, we test the accuracy of AcGGM within the complex endogenous gut microbiome of pigs, wherein we resolve 355 metagenome-assembled genomes together with quantitative metaproteomes. In AcGGM-fed pigs, both target populations differentially express AcGGM-specific polysaccharide utilization loci, including novel, mannan-specific esterases that are critical to its deconstruction. However, AcGGM-inclusion also manifests a “butterfly effect”, whereby numerous metabolic changes and interdependent cross-feeding pathways occur in neighboring non-mannanolytic populations that produce short-chain fatty acids. Our findings show how intricate structural features and acetylation patterns of dietary fibre can be customized to specific bacterial populations, with potential to create greater modulatory effects at large.
Author
Leszek Michalak
Norwegian University of Life Sciences
John Christian Gaby
Norwegian University of Life Sciences
Leidy Lagos
Norwegian University of Life Sciences
Sabina Leanti La Rosa
Norwegian University of Life Sciences
Torgeir R. Hvidsten
Norwegian University of Life Sciences
Catherine Tétard-Jones
Newcastle University
William G.T. Willats
Newcastle University
Nicolas Terrapon
National Institute of Agronomic Research (INRA)
Aix Marseille University
Vincent Lombard
National Institute of Agronomic Research (INRA)
Aix Marseille University
B. Henrissat
King Abdulaziz University
Aix Marseille University
National Institute of Agronomic Research (INRA)
Johannes Dröge
Chalmers, Mathematical Sciences, Applied Mathematics and Statistics
Magnus Arntzen
Norwegian University of Life Sciences
Live Heldal Hagen
Norwegian University of Life Sciences
Margareth Øverland
Norwegian University of Life Sciences
P. B. Pope
Norwegian University of Life Sciences
Bjørge Westereng
Norwegian University of Life Sciences
Nature Communications
2041-1723 (ISSN) 20411723 (eISSN)
Vol. 11 1 5773Subject Categories
Evolutionary Biology
Other Biological Topics
Genetics
DOI
10.1038/s41467-020-19585-0
PubMed
33188211