Herpes simplex virus type 2 mucin-like glycoprotein mgg promotes virus release from the surface of infected cells
Journal article, 2021

The contribution of virus components to liberation of herpes simplex virus type 2 (HSV-2) progeny virions from the surface of infected cells is poorly understood. We report that the HSV-2 mutant deficient in the expression of a mucin-like membrane-associated glycoprotein G (mgG) exhibited defect in the release of progeny virions from infected cells manifested by ~2 orders of magnitude decreased amount of infectious virus in a culture medium as compared to native HSV-2. Electron microscopy revealed that the mgG deficient virions were produced in infected cells and present at the cell surface. These virions could be forcibly liberated to a nearly native HSV-2 level by the treatment of cells with glycosaminoglycan (GAG)-mimicking oligosaccharides. Comparative assessment of the interaction of mutant and native virions with surface-immobilized chondroitin sulfate GAG chains revealed that while the mutant virions associated with GAGs ~fourfold more extensively, the lateral mobility of bound virions was much poorer than that of native virions. These data indicate that the mgG of HSV-2 balances the virus interaction with GAG chains, a feature critical to prevent trapping of the progeny virions at the surface of infected cells.


single particle analysis

herpes simplex virus type 2

mucin-like protein

glycoprotein mgG

virus release


Edward Trybala

University of Gothenburg

Nadia Peerboom

Chalmers, Physics, Biological Physics

Beata Adamiak

University of Gothenburg

Malgorzata Krzyzowska

University of Gothenburg

Jan Åke Liljeqvist

University of Gothenburg

Marta Bally

Umeå University

Tomas Bergström

University of Gothenburg


1999-4915 (ISSN) 19994915 (eISSN)

Vol. 13 5 887

Subject Categories

Cell Biology

Cell and Molecular Biology

Immunology in the medical area



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