A gene expression fingerprint of mouse stomach ECL cells.
Journal article, 2005

Many of the endocrine cells in the stomach are poorly characterized with respect to physiological significance. In some cases, the anticipated hormone has not yet been identified. Global gene expression analysis of mouse stomach was performed in an attempt to identify the ECL-cell peptide/protein. Specific functional activation (omeprazole-induced hypergastrinaemia) was used as a tool to generate a gene expression fingerprint of the ECL cells. The proposed fingerprint includes 14 genes, among them six are known to be expressed by ECL cells (=positive controls), and some novel ones, which are likely to be ECL-cell-related. The known ECL-cell-related genes are those encoding histidine decarboxylase, chromogranin A and B, vesicular monoamine transporter 2, synaptophysin, and the cholecystokinin-B receptor. In addition, the fingerprint included five genes, which might be involved in the process of secretion and three ESTs with unknown function. Interestingly, parathyroid hormone-like hormone (Pthlh) was identified as a candidate ECL-cell peptide hormone.

Stomach

metabolism

metabolism

Female

Animals

chemically induced

Tissue Distribution

metabolism

metabolism

Omeprazole

Mice

Gastrins

Enteroendocrine Cells

Cultured

Proteome

Gastric Mucosa

metabolism

Stomach Diseases

Cells

Inbred C57BL

metabolism

methods

Peptide Mapping

metabolism

Peptide Hormones

Mice

Author

Niklas Andersson

University of Gothenburg

Sofia Movérare-Skrtic

University of Gothenburg

Rolf Håkanson

Claes Ohlsson

University of Gothenburg

Biochemical and Biophysical Research Communications

0006-291X (ISSN) 1090-2104 (eISSN)

Vol. 332 2 404-10

Subject Categories

MEDICAL AND HEALTH SCIENCES

DOI

10.1016/j.bbrc.2005.05.004

PubMed

15896709

More information

Created

10/10/2017