Chemical imaging of lignocellulosic biomass by CARS microscopy
Journal article, 2014

Chemical and structural composition of wood biomass is studied by label-free and chemically specific Coherent Anti-Stokes Raman Scattering (CARS) microscopy. A concept developed for assignment and semi-quantitative imaging of sample components; cellulose, hemicellulose, and lignin; by multiplex CARS microspectroscopy and subsequent data analysis is presented. Specific imaging without fluorescence backround is achieved an order of magnitude faster compared with conventional Raman microscopy. Laser polarization control yield information on molecular arrangement in wood fibers. Narrowband CARS excitation of single vibrations allows for three-dimensional volume imaging. Thus, CARS microscopy has potential as an important instrument for characterization of lignocellulosic materials.

cellulose

wood

lignin

biomass

CARS microscopy

xylan

Author

C. Pohling

Heidelberg University

Christian Brackmann

Chalmers, Chemical and Biological Engineering, Molecular Imaging

A. Duarte

Heidelberg University

T. Buckup

Heidelberg University

Annika Enejder

Chalmers, Chemical and Biological Engineering, Molecular Imaging

M. Motzkus

Heidelberg University

Journal of Biophotonics

1864-063X (ISSN) 18640648 (eISSN)

Vol. 7 1-2 126-134

Subject Categories

Physical Chemistry

DOI

10.1002/jbio.201300052

More information

Latest update

4/20/2018