Livsmedelsingredienser från CO2 med mikrobiella konsortia

Research Project, 2022 – 2026

We develop a light-powered microbial consortia to convert CO2 to tailored food additives. Through extensive molecular breeding, enzyme engineering, and high-throughput screening, we will create a platform strain of the yeast S. cerevisiae to accumulate palm and coconut oils, two growing markets with unsustainable supply. To provide additional value, we will generate strain agnostic, designer enzymes for valorizing fatty-acids into other food additives. In parallel, we will optimize a “division of labor,” co-culture, where CO2 is fixed by photosynthetic cyanobacteria and secreted as sugar to yeast. Cyanobacteria will be enhanced using machine-learning guided mutation of the Calvin cycle. The combinatorial screening of culture parameters and genetic mutations is achieved with novel microfluidics technology. The immediate outcome will be novel strains and enzymes with high industrial potential. By focusing on S. cerevisiae, we build upon previous success in production of fats, and access unique synthetic biology tools. The interdisciplinary nature will give our results a broad reach and high impact. Our implementation plan includes licensing IP to Biopetrolia, a Chalmers spinout. Our reference board, with representatives from 4 Swedish companies, will help us reach industrial stakeholders, identify applications for results, and advise trainees. A long-term vision is a viable fermentation platform in Sweden that replaces import of myriad food ingredients derived from plants.