Tracking cellular nanomotions with optically controlled rotary nanomotors

Research Project, 2024 – 2027

Motion is a fundamental feature of living organisms, regardless of their size. Recently, nanoscale motions of single cells have been correlated with cell viability and health. However, existing measurement techniques have limitations in resolution and sensitivity, preventing the precise capture of subcellular motion and limiting the understanding of the relationship between cellular nanomotion and specific cellular functions or pathologies. To address this gap, this project aims to develop a highly precise and minimally invasive optical technique based on optically driven rotating gold nanoparticles that can measure the nanomotions of single living cells. Through this novel approach, we seek to investigate the relationship between cellular metabolism and nanomotion amplitude and frequency. Additionally, by simultaneously monitoring multiple areas on the cellular surface, we will determine the source, amplitude, wavelength, and direction of mechanical waves generated by cellular processes. We will also examine the potential applications of detecting and characterizing nanomotions in single bacteria cells, including rapid antibiotic susceptibility testing. Overall, this proposed research is expected to provide valuable insights into cellular dynamics and functions and have a significant impact on the development of new diagnostic tools, personalized treatments, and therapies targeting defects in cellular processes.