Yeast-based biosensors for the specific and accessible detection of pathogens and antimicrobial resistance

Research Project, 2019 – 2021

Early and specific detection of microbial infections is crucial for the containment of diseases and for reducing the dependence on the use of antibiotics. There is however a lack of reliable, cheap and easy to use detection methods for day-to-day monitoring of infection and antimicrobial resistance in samples from patients, animals and the environment. This deficiency is critical for the abuse of antibiotics and the diffusion of antimicrobial resistance. The aim of this project is to establish a method based on yeast biosensors that can detect with high specificity pathogens from different sources to develop a new fast diagnostic tool for resistant pathogens as well as specific bacterial species. We will achieve this by joining together strong research groups on antimicrobial resistance, systems biology, and strain engineering at SINTEF, Chalmers University and National Medicines Institute. Particular focus will be given to the detection of ESBL or carbapenemase-producing strains belonging to the emerging ESKAPE group of resistant pathogens. The biosensor is developed using yeast as host to express specific receptors able to recognise unique molecules produced by the pathogens. The ligand-receptor binding initiates a cascade mechanism that activates the genes for the production of a red pigment. Using the biosensors, we aim to identify molecular markers specific for resistant pathogen strains, to enable fast, easy and inexpensive point-of-use profiling of resistant pathogens.