Quantitative chemical analysis of single cells.
Book chapter, 2009
Exocytosis, the fusion of intracellular vesicles with the membrane and subsequent release of vesicular contents, is important in intercellular communication. The release event is a rapid process (milliseconds), hence detection of released chemicals requires a detection scheme that is both sensitive and has rapid temporal dynamics. Electrochemistry at carbon-fiber microelectrodes allows time-resolved exocytosis of electroactive catecholamines to be observed at very low levels. When coupled with constant-potential amperometry, the number of molecules released and the kinetics of quantal release can be determined. The rapid response time (milliseconds) of microelectrodes makes them well suited for monitoring the dynamic process of exocytosis.
Cells
methods
chemistry
Electrochemical Techniques
Animals
Rats
Exocytosis
secretion
PC12 Cells
instrumentation
Microelectrodes
Author
M. L. Heien
Andrew Ewing
University of Gothenburg
Methods in molecular biology book series, Volume 544, Part 2: Micro and Nano Technologies in Bioanalysis (eds Robert S. Foote, James Weifu Lee),
153-62
978-1-59745-483-4 (ISBN)
Subject Categories
Chemical Sciences
DOI
10.1007/978-1-59745-483-4_11
PubMed
19488699
ISBN
978-1-59745-483-4