Quantitative chemical analysis of single cells.
Kapitel i bok, 2009

Exocytosis, the fusion of intracellular vesicles with the membrane and subsequent release of vesicular contents, is important in intercellular communication. The release event is a rapid process (milliseconds), hence detection of released chemicals requires a detection scheme that is both sensitive and has rapid temporal dynamics. Electrochemistry at carbon-fiber microelectrodes allows time-resolved exocytosis of electroactive catecholamines to be observed at very low levels. When coupled with constant-potential amperometry, the number of molecules released and the kinetics of quantal release can be determined. The rapid response time (milliseconds) of microelectrodes makes them well suited for monitoring the dynamic process of exocytosis.

Cells

methods

chemistry

Electrochemical Techniques

Animals

Rats

Exocytosis

secretion

PC12 Cells

instrumentation

Microelectrodes

Författare

M. L. Heien

Andrew Ewing

Göteborgs universitet

Methods in molecular biology book series, Volume 544, Part 2: Micro and Nano Technologies in Bioanalysis (eds Robert S. Foote, James Weifu Lee),

153-62

Ämneskategorier

Kemi

DOI

10.1007/978-1-59745-483-4_11

PubMed

19488699

ISBN

978-1-59745-483-4