Andrew Ewing

2022

Visualization of Partial Exocytotic Content Release and Chemical Transport into Nanovesicles in Cells

2021

Striatal Dopamine Transporter Function Is Facilitated by Converging Biology of α-Synuclein and Cholesterol

2020

Subcellular Mass Spectrometry Imaging and Absolute Quantitative Analysis across Organelles

2020

Interplay between Cocaine, Drug Removal, and Methylphenidate Reversal on Phospholipid Alterations in Drosophila Brain Determined by Imaging Mass Spectrometry

2020

Mass spectrometric imaging of plasma membrane lipid alteration correlated with amperometrically measured activity-dependent plasticity in exocytosis

Altered Lipid Composition of Secretory Cells Following Exposure to Zinc Can Be Correlated to Changes in Exocytosis

Combined Amperometry and Electrochemical Cytometry Reveal Differential Effects of Cocaine and Methyphenidate on Exocytosis and the Fraction of Chemical Release

Mass spectrometry imaging as a novel approach to measure hippocampal zinc

Nanopore Opening at Flat and Nanotip Conical Electrodes during Vesicle Impact Electrochemical Cytometry

Mass Spectrometry Imaging Shows Cocaine and Methylphenidate Have Opposite Effects on Major Lipids in Drosophila Brain

On the Action of General Anesthetics on Cellular Function: Barbiturate Alters the Exocytosis of Catecholamines in a Model Cell System

Mass Spectrometry Imaging Suggests That Cisplatin Affects Exocytotic Release by Alteration of Cell Membrane Lipids

Using Single-Cell Amperometry and Intracellular Vesicle Impact Electrochemical Cytometry to Shed Light on the Biphasic Effects of Lidocaine on Exocytosis

On-Tissue Chemical Derivatization of Catecholamines Using 4-(N-Methyl)pyridinium Boronic Acid for ToF-SIMS and LDI-ToF Mass Spectrometry Imaging

Electrochemistry in and of the Fly Brain

Benefits of NaCl addition for time-of-flight secondary ion mass spectrometry analysis including the discrimination of diacylglyceride and triacylglyceride ions

Electrochemical quantification of transmitter concentration in single nanoscale vesicles isolated from PC12 cells

Relative quantification of deuterated omega-3 and -6 fatty acids and their lipid turnover in PC12 cell membranes using TOF-SIMS

Electrochemical Investigation of the Interaction between Catecholamines and ATP

Measuring synaptic vesicles using cellular electrochemistry and nanoscale molecular imaging

Ultrasonic-Aided Fabrication of Nanostructured Au-Ring Microelectrodes for Monitoring Transmitters Released from Single Cells

Mechanistic Aspects of Vesicle Opening during Analysis with Vesicle Impact Electrochemical Cytometry

Vesicle impact electrochemical cytometry compared to amperometric exocytosis measurements

DMSO Chemically Alters Cell Membranes to Slow Exocytosis and Increase the Fraction of Partial Transmitter Released

Zinc Regulates Chemical-Transmitter Storage in Nanometer Vesicles and Exocytosis Dynamics as Measured by Amperometry

Nano Secondary Ion Mass Spectrometry Imaging of Dopamine Distribution Across Nanometer Vesicles

MS/MS analysis and imaging of lipids across Drosophila brain using secondary ion mass spectrometry

2016

Cell Analysis with Bioelectrochemical Approaches: Past, Present, and Future

From single cells to single molecules: general discussion

Lithographic Microfabrication of a 16-Electrode Array on a Probe Tip for High Spatial Resolution Electrochemical Localization of Exocytosis

Using Single-Cell Amperometry To Reveal How Cisplatin Treatment Modulates the Release of Catecholamine Transmitters during Exocytosis

2016

Food-induced changes of lipids in rat neuronal tissue visualized by ToF-SIMS imaging

Single cell amperometry reveals curcuminoids modulate the release of neurotransmitters during exocytosis from PC12 cells

Cysteine residues reduce the severity of dopamine electrochemical fouling

Mass spectrometric profiling of lipids in intestinal tissue from rats fed cereals processed for medical conditions

Multimodal Imaging of Chemically Fixed Cells in Preparation for NanoSIMS

Mass spectrometry imaging of lipids and metabolites: from fruit fly brains to single nanometer vesicles

Laser Desorption Ionization Mass Spectrometry Imaging of Drosophila Brain Using Matrix Sublimation versus Modification with Nanoparticles

Intact lipid imaging of mouse brain samples: MALDI, nanoparticle-laser desorption ionization, and 40 keV argon cluster secondary ion mass spectrometry

The evidence for open and closed exocytosis as the primary release mechanism

On the mechanism of electrochemical vesicle cytometry: chromaffin cell vesicles and liposomes

An investigation on the mechanism of sublimed DHB matrix on molecular ion yields in SIMS imaging of brain tissue

Excited Fluorophores Enhance the Opening of Vesicles at Electrode Surfaces in Vesicle Electrochemical Cytometry

Quantitative Chemical Measurements of Vesicular Transmitters with Electrochemical Cytometry

Quantitative Measurement of Transmitters in Individual Vesicles in the Cytoplasm of Single Cells with Nanotip Electrodes

Selected recent in vivo studies on chemical measurements in invertebrates

Lipid Structural Effects of Oral Administration of Methylphenidate in Drosophila Brain by Secondary Ion Mass Spectrometry Imaging

Neuronal Networks on Nanocellulose Scaffolds

Spatial neuroproteomics using imaging mass spectrometry

The Effect of Excited Fluorophore on Vesicle Fusion at the Surface of the Electrode

Characterizing the Catecholamine Content of Single Mammalian Vesicles by Collision-Adsorption Events at an Electrode

Electrochemical Measurements of Optogenetically Stimulated Quantal Amine Release from Single Nerve Cell Varicosities in Drosophila Larvae

Imaging Mass Spectrometry for Single-Cell Analysis

Probing the lipid chemistry of neurotoxin-induced hippocampal lesions using multimodal imaging mass spectrometry

ToF-SIMS imaging of lipids and lipid related compounds in Drosophila brain

Applying THz Technology in Life Science

Development of an Organic Lateral Resolution Test Device for Imaging Mass Spectrometry

2014

Two modes of exocytosis in an artificial cell

Using imaging ToF-SIMS data to determine the cell wall thickness of fibers in wood

Spatial Resolution of Single-Cell Exocytosis by Microwell-Based Individually Addressable Thin Film Ultramicroelectrode Arrays

Analysis of liposome model systems by time-of-flight secondary ion mass spectrometry

Gold and silver nanoparticle-assisted laser desorption ionization mass spectrometry compatible with secondary ion mass spectrometry for lipid analysis

Pituitary Adenylate Cyclase Activating Polypeptide Modulates Catecholamine Storage and Exocytosis in PC12 Cells

Simultaneous study of subcellular exocytosis with individually addressable multiple microelectrodes

High Resolution Metabolite Imaging in the Hippocampus Following Neonatal Exposure to the Environmental Toxin BMAA Using ToF-SIMS

Actin Controls the Vesicular Fraction of Dopamine Released During Extended Kiss and Run Exocytosis

2014

Spatial Elucidation of Spinal Cord Lipid- and Metabolite-Regulations in Amyotrophic Lateral Sclerosis

Individually Addressable Thin-Film Ultramicroelectrode Array for Spatial Measurements of Single Vesicle Release

Single Cell Amperometry Reveals Glycocalyx Hinders the Release of Neurotransmitters During Exocytosis

Time-of-Flight Secondary Ion Mass Spectrometry Based Molecular Histology of Human Spinal Cord Tissue and Motor Neurons

Freeze-Drying as Sample Preparation for Micellar Electrokinetic Capillary Chromatography-Electrochemical Separations of Neurochemicals in Drosophila Brains

Electrochemical measurements of transmitters in flies, at cells, and from transmitter vesicles

C-60-SIMS studies of glycerophospholipid in a LIPID MAPS model system: KDO2-Lipid A stimulated RAW 264.7 cells

Capillary Electrophoresis-Mass Spectrometry-Based Detection of Drugs and Neurotransmitters in Drosophila Brain

Chemical Analysis of Single Cells

Single-Cell Lipidomics: Characterizing and Imaging Lipids on the Surface of Individual Aplysia californica Neurons with Cluster Secondary Ion Mass Spectrometry

Oral administration of methylphenidate blocks the effect of cocaine on uptake at the Drosophila dopamine transporter.

Imaging mass spectrometry in neuroscience.

Amperometric Measurements at Cells Support a Role for Dynamin in the Dilation of the Fusion Pore during Exocytosis

C60-ToF SIMS imaging of frozen hydrated HeLa cells

2013

The real catecholamine content of secretory vesicles in the CNS revealed by electrochemical cytometry

Mass spectrometry imaging of freeze-dried membrane phospholipids of dividing Tetrahymena pyriformis

Carbon Nanotube Fiber Microelectrodes Show a Higher Resistance to Dopamine Fouling

Spatial resolution of exocytosis across a single cell by a microwell-based individually addressable thin film ultra-microelectrode array

Evaluating the Diffusion Coefficient of Dopamine at the Cell Surface During Amperometric Detection: Disk vs Ring Microelectrodes

2012

Amperometric post spike feet reveal most exocytosis is via extended kiss-and-run fusion

Analytical tools to monitor exocytosis: A focus on new fluorescent probes and methods

Highlights of selected recent electrochemical measurements in living systems

Carbon-Ring Microelectrode Arrays for Electrochemical Imaging of Single Cell Exocytosis: Fabrication and Characterization

Chemical Analysis of Single Cells

Ruthenium(II) Complex Enantiomers as Cellular Probes for Diastereomeric Interactions in Confocal and Fluorescence Lifetime Imaging Microscopy

Highlights of 20 years of electrochemical measurements of exocytosis at cells and artificial cells

Temporal Resolution in Electrochemical Imaging on Single PC12 Cells Using Amperometry and Voltammetry at Microelectrode Arrays

Relative quantification of phospholipid accumulation in the PC12 cell plasma membrane following phospholipid incubation using TOF-SIMS Imaging.

2011

Mechanics of lipid bilayer junctions affecting the size of a connecting lipid nanotube

An in situ fracture device to image lipids in single cells using ToF-SIMS

Micellar capillary electrophoresis - Electrochemical detection of neurochemicals from Drosophila

Steady-State Electrochemical Determination of Lipidic Nanotube Diameter Utilizing an Artificial Cell Model

Using in Vivo Electrochemistry To Study the Physiological Effects of Cocaine and Other Stimulants on the Drosophila melanogaster Dopamine Transporter

Mass spectrometry imaging of mating Tetrahymena show that changes in cell morphology regulate lipid domain formation

Biogenic Amines in Microdissected Brain Regions of Drosophila melanogaster Measured with Mice liar Electrokinetic Capillary Chromatography-Electrochemical Detection

Probing Exocytosis at Single Cells using Electrochemistry

Electrochemical Probes for Spatial Detection of Exocytosis and Vesicles

Nanotome Cluster Bombardment to Recover Spatial Chemistry After Preparation of Biological Samples for SIMS Imaging

Analytical approaches to investigate transmitter content and release from single secretory vesicles

Development and Characterization of a Voltammetric Carbon-Fiber Microelectrode pH Sensor

Time of Flight Mass Spectrometry Imaging of Samples Fractured In Situ with a Spring-Loaded Trap System.

Determination of salsolinol, norsalsolinol, and twenty-one biogenic amines using micellar electrokinetic capillary chromatography-electrochemical detection

Only a Fraction of Quantal Content is Released During Exocytosis as Revealed by Electrochemical Cytometry of Secretory Vesicles

Tools to monitor exocytosis: a focus on new fluorescent probes and methods

Quantitative chemical analysis of single cells.

In Vivo Electrochemical Measurements of Exogenously Applied Dopamine in Drosophila melanogaster.

Hybrid capillary-microfluidic device for the separation, lysis, and electrochemical detection of vesicles.

Chemical measurements in Drosophila

Time-of-flight secondary ion mass spectrometry imaging of subcellular lipid heterogeneity: Poisson counting and spatial resolution.

Temporal analysis of protozoan lysis in a microfluidic device.

Inhibition of HMGCoA Reductase Reveals An Unexpected Role for Cholesterol During PGC Migration in the Mouse.

Short-Chain Alcohols Promote Accelerated Membrane Distention in a Dynamic Liposome Model of Exocytosis

Spatially and Temporally Resolved Single-Cell Exocytosis Utilizing Individually Addressable Carbon Microelectrode Arrays

Investigating lipid–lipid and lipid–protein interactions in model membranes by ToF-SIMS

Amperometric measurements of catecholamine release from single vesicles in MN9D cells.

Which is more important in bioimaging SIMS experiments-The sample preparation or the nature of the projectile?

Relative quantification of cellular sections with molecular depth profiling ToF-SIMS imaging

Freeze-Etching and Vapor Matrix Deposition for ToF-SIMS Imaging of Single Cells

The PC12 cell as model for neurosecretion

In Vitro Electrochemistry of Biological Systems

MS/MS Methodology To Improve Subcellular Mapping of Cholesterol Using TOF-SIMS

Flow characterization of a microfluidic device to selectively and reliably apply reagents to a cellular network

Electrochemistry in and at single biological cells

Recent advances in capillary electrophoretic analysis of individual cells

High Osmolarity and L-DOPA Augment Release via the Fusion Pore in PC12 Cells

Electrochemistry inside and outside single nerve cells

Capillary Electrophoresis for the Analysis of Single Cells: Sampling, Detection and Applications

Localization of Sphingomyelin in Cholesterol Domains by Imaging Mass Spectrometry

Multicore Vesicles: Hyperosmolarity and l-DOPA Induce Homotypic Fusion of Dense Core Vesicles

Phospholipid mediated plasticity in exocytosis observed in PC12 cells

Secondary Ion MS Imaging To Relatively Quantify Cholesterol in the Membranes of Individual Cells from Differentially Treated Populations

Electrochemistry at the cell membrane/solution interface

The effects of vesicular volume on secretion through the fusion pore in exocytotic release from PC12 cells

Measurement of the Dynamics of Exocytosis and Vesicle Retrieval at Cell Populations using a Quartz Crystal Microbalance