Using Single-Cell Amperometry To Reveal How Cisplatin Treatment Modulates the Release of Catecholamine Transmitters during Exocytosis
Artikel i vetenskaplig tidskrift, 2016

The pretreatment of cultured pheochromocytoma (PC12) cells with cis-diamminedichloroplatinum (cisplatin), an anti-cancer drug, influences the exocytotic ability of the cells in a dose-dependent manner. Low concentrations of cisplatin stimulate catecholamine release whereas high concentrations inhibit it. Single-cell amperometry reflects that 2 mm cisplatin treatment increases the frequency of exocytotic events and reduces their duration, whereas 100 mm cisplatin treatment decreases the frequency of exocytotic events and increases their duration. Furthermore, the stability of the initial fusion pore that is formed in the lipid membrane during exocytosis is also regulated differentially by different cisplatin concentrations. This study thus suggests that cisplatin influences exocytosis by multiple mechanisms.

exocytosis

chemotherapy

cisplatin

amperometry

Författare

Li Xianchan

Göteborgs universitet

Johan Dunevall

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Andrew Ewing

Göteborgs universitet

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Angewandte Chemie - International Edition

1433-7851 (ISSN) 1521-3773 (eISSN)

Vol. 55 9041-9044

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1002/anie.201602977