Excited Fluorophores Enhance the Opening of Vesicles at Electrode Surfaces in Vesicle Electrochemical Cytometry
Artikel i vetenskaplig tidskrift, 2016

Electrochemical cytometry is a method developed recently to determine the content of an individual cell vesicle. The mechanism of vesicle rupture at the electrode surface involves the formation of a pore at the interface between a vesicle and the electrode through electroporation, which leads to the release and oxidation of the vesicle's chemical cargo. We have manipulated the membrane properties using excited fluorophores conjugated to lipids, which appears to make the membrane more susceptible to electroporation. We propose that by having excited fluorophores in close contact with the membrane, membrane lipids (and perhaps proteins) are oxidized upon production of reactive oxygen species, which then leads to changes in membrane properties and the formation of water defects. This is supported by experiments in which the fluorophores were placed on the lipid tail instead of the headgroup, which leads to a more rapid onset of vesicle opening. Additionally, application of DMSO to the vesicles, which increases the membrane area per lipid, and decreasing the membrane thickness result in the same enhancement in vesicle opening, which confirms the mechanism of vesicle opening with excited fluorophores in the membrane. Light-induced manipulation of membrane vesicle pore opening might be an attractive means of controlling cell activity and exocytosis. Additionally, our data confirm that in experiments in which cells or vesicle membranes are labeled for fluorescence monitoring, the properties of the excited membrane change substantially.

electroporation

vesicles

fusion pores

chemical cytometry

fluorophores

Författare

Neda Najafinobar

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Jelena Lovric

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Soodabeh Majdi

Göteborgs universitet

Johan Dunevall

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Ann-Sofie Cans

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Andrew Ewing

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Angewandte Chemie - International Edition

1433-7851 (ISSN) 1521-3773 (eISSN)

Vol. 55 48 15081-15085

Ämneskategorier

Fysikalisk kemi

DOI

10.1002/anie.201609178

Mer information

Senast uppdaterat

2018-02-21