Ann-Sofie Cans

Artificial Cells for Dissecting Exocytosis

2020

Generation of interconnected vesicles in a liposomal cell model

Molecular Crowding and a Minimal Footprint at a Gold Nanoparticle Support Stabilize Glucose Oxidase and Boost Its Activity

Electrochemistry of Single-Vesicle Events

Ultrafast Glutamate Biosensor Recordings in Brain Slices Reveal Complex Single Exocytosis Transients

Counting the Number of Glutamate Molecules in Single Synaptic Vesicles

2018

Co-detection of dopamine and glucose with high temporal resolution

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

2018

Counting the number of enzymes immobilized onto a nanoparticle-coated electrode

Monitoring the effect of osmotic stress on secretory vesicles and exocytosis

2018

Amperometry methods for monitoring vesicular quantal size and regulation of exocytosis release

Extracellular Osmotic Stress Reduces the Vesicle Size while Keeping a Constant Neurotransmitter Concentration

Lithographic Microfabrication of a 16-Electrode Array on a Probe Tip for High Spatial Resolution Electrochemical Localization of Exocytosis

The evidence for open and closed exocytosis as the primary release mechanism

Excited Fluorophores Enhance the Opening of Vesicles at Electrode Surfaces in Vesicle Electrochemical Cytometry

2016

Cholesterol Alters the Dynamics of Release in Protein Independent Cell Models for Exocytosis

Development of Ultra-Fast Nanostructured Glucose Biosensor - Stoichiometry and Activity

Millisecond Time Resolved Electrochemical Detection of Non-Electroactive Neurotransmitter Release

Amperometric Detection of Single Vesicle Acetylcholine Release Events from an Artificial Cell

The Effect of Excited Fluorophore on Vesicle Fusion at the Surface of the Electrode

Characterizing the Catecholamine Content of Single Mammalian Vesicles by Collision-Adsorption Events at an Electrode

Using a Cell Model to Study the Effect of Cholesterol on Exocytosis

2014

Two modes of exocytosis in an artificial cell

Electrochemcial Detection of Acetylcholine using Enzyme Functionalized Nanoparticles

2014

Composition Based Strategies for Controlling Radii in Lipid Nanotubes

Coimmobilization of Acetylcholinesterase and Choline Oxidase on Gold Nanoparticles: Stoichiometry, Activity, and Reaction Efficiency

The Role of Dense Core Nanoparticles in Regulation of Neuronal Communication

A Secretory Artificial Cell for Exocytosis

2013

The real catecholamine content of secretory vesicles in the CNS revealed by electrochemical cytometry

Analytical tools to monitor exocytosis: A focus on new fluorescent probes and methods

Highlights of selected recent electrochemical measurements in living systems

2012

A functioning artificial secretory cell

Carbon-Ring Microelectrode Arrays for Electrochemical Imaging of Single Cell Exocytosis: Fabrication and Characterization

Highlights of 20 years of electrochemical measurements of exocytosis at cells and artificial cells

2011

Mechanics of lipid bilayer junctions affecting the size of a connecting lipid nanotube

Steady-State Electrochemical Determination of Lipidic Nanotube Diameter Utilizing an Artificial Cell Model

Electrochemical Probes for Spatial Detection of Exocytosis and Vesicles

Analytical approaches to investigate transmitter content and release from single secretory vesicles

Tools to monitor exocytosis: a focus on new fluorescent probes and methods

Budding and asymmetric protein microcompartmentation in giant vesicles containing two aqueous phases

Positioning lipid membrane domains in giant vesicles by micro-organization of aqueous cytoplasm mimic

The effects of vesicular volume on secretion through the fusion pore in exocytotic release from PC12 cells

Micropipet-assisted formation of microscopic networks of unilamellar lipid bilayer nanotubes and containers

Measurement of the Dynamics of Exocytosis and Vesicle Retrieval at Cell Populations using a Quartz Crystal Microbalance