Electrochemical Probes for Spatial Detection of Exocytosis and Vesicles
Artikel i vetenskaplig tidskrift, 2010

Unraveling the mechanistic details of neurotransmitter exocytosis is arguably among the most important molecular problems in neuroscience today. Investigations at single cells, particularly with electrochemical methods, have given unique chemical and biological insight into this process at the fundamental level. The rapid response time (submillisecond) of microelectrodes makes them well suited for monitoring the dynamic process of exocytosis. We review here recent developments in electrochemical techniques to spatially and simultaneously detect exocytosis across a single cell and to measure the transmitter content of single vesicles removed from cells. The former method is used to demonstrate dynamic heterogeneity in release across a cell, and in the latter work comparison is made between vesicle content and release to conclude that only a fraction of the transmitter is released during full exocytosis.

cytometry

patch clamp technique

exocytosis

vesicles

electrochemistry

Författare

Lisa Mellander

Göteborgs universitet

Ann-Sofie Cans

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Andrew Ewing

Göteborgs universitet

ChemPhysChem

1439-4235 (ISSN) 1439-7641 (eISSN)

Vol. 11 2756-2763

Ämneskategorier

Analytisk kemi

Neurovetenskaper

DOI

10.1002/cphc.201000258