A functioning artificial secretory cell
Artikel i vetenskaplig tidskrift, 2012

We present an amperometric study of content release from individual vesicles in an artificial secretory cell designed with the minimal components required to carry out exocytosis. Here, the membranes of the cell and vesicles are substituted for protein-free giant and large unilamellar vesicles respectively. In replacement of the SNARE-complex, the cell model was equipped with an analog composed of complimentary DNA constructs. The DNA constructs hybridize in a zipper-like fashion to bring about docking of the artificial secretory vesicles and following the addition of Ca2+ artificial exocytosis was completed. Exocytotic events recorded from the artificial cell closely approximate exocytosis in live cells. The results together with simulations of vesicular release demonstrate that the molecular flux in this model is attenuated and we suggest that this is the result of restricted diffusion through a semi-stable fusion pore or a partitioning of the signalling molecule out of the fused vesicle membrane.

amperometry

vesicle fusion

dopamine

chromaffin cells

lipid-bilayer

nanotubes

release

exocytotic events

pc12 cells

membrane-fusion

Författare

Lisa Simonsson Nyström

Chalmers, Teknisk fysik, Biologisk fysik

Michael Kurczy

Chalmers, Kemi- och bioteknik, Analytisk kemi

R. Trouillon

Göteborgs universitet

Fredrik Höök

Chalmers, Teknisk fysik, Biologisk fysik

Ann-Sofie Cans

Chalmers, Kemi- och bioteknik, Analytisk kemi

Scientific Reports

2045-2322 (ISSN)

Vol. 2 no. 824- 824

Hydrodynamisk koncentrering av native membranprotein för funktionella studier på chip format

Vetenskapsrådet (VR), 2011-01-01 -- 2014-12-31.

Ämneskategorier

Biokemi och molekylärbiologi

Analytisk kemi

Biofysik

DOI

10.1038/srep00824