Ultrafast Glutamate Biosensor Recordings in Brain Slices Reveal Complex Single Exocytosis Transients
Artikel i vetenskaplig tidskrift, 2019

Neuronal communication relies on vesicular neurotransmitter release from signaling neurons and detection of these molecules by neighboring neurons. Glutamate, the main excitatory neurotransmitter in the mammalian brain, is involved in nearly all brain functions. However, glutamate has suffered from detection schemes that lack temporal and spatial resolution allowed by electrochemistry. Here we show an amperometric, novel, ultrafast enzyme-based nanoparticle modified sensor, measuring random bursts of hundreds to thousands of rapid spontaneous glutamate exocytotic release events at approximately 30 Hz frequency in the nucleus accumbens of rodent brain slices. Characterizing these single submillisecond exocytosis events revealed a great diversity in spike shape characteristics and size of quantal release, suggesting variability in fusion pore dynamics controlling the glutamate release by cells in this brain region. Hence, this novel biosensor allows recording of rapid single glutamate exocytosis events in the brain tissue and offers insight on regulatory aspects of exocytotic glutamate release, which is critical to understanding of brain glutamate function and dysfunction.

microelectrode

rodent

fusion pore

amperometry

glutamate oxidase

brain slice

nucleus accumbens

gold nanoparticle

ultrafast

biosensor

Glutamate

exocytosis

dynamics

Författare

Yuanmo Wang

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Devesh Mishra

Wallenberg Lab.

Sahlgrenska akademin

Jenny Bergman

Göteborgs universitet

Jacqueline Keighron

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Analytisk kemi

Karolina P. Skibicka

Sahlgrenska akademin

Wallenberg Lab.

Ann-Sofie Cans

Chalmers, Kemi och kemiteknik, Kemi och biokemi

ACS Chemical Neuroscience

1948-7193 (ISSN)

Vol. 10 3 1744-1752

Ämneskategorier

Cellbiologi

Annan medicinteknik

Neurovetenskaper

DOI

10.1021/acschemneuro.8b00624

PubMed

30605606

Mer information

Senast uppdaterat

2019-04-29