Ultrafast Glutamate Biosensor Recordings in Brain Slices Reveal Complex Single Exocytosis Transients
Journal article, 2019

Neuronal communication relies on vesicular neurotransmitter release from signaling neurons and detection of these molecules by neighboring neurons. Glutamate, the main excitatory neurotransmitter in the mammalian brain, is involved in nearly all brain functions. However, glutamate has suffered from detection schemes that lack temporal and spatial resolution allowed by electrochemistry. Here we show an amperometric, novel, ultrafast enzyme-based nanoparticle modified sensor, measuring random bursts of hundreds to thousands of rapid spontaneous glutamate exocytotic release events at approximately 30 Hz frequency in the nucleus accumbens of rodent brain slices. Characterizing these single submillisecond exocytosis events revealed a great diversity in spike shape characteristics and size of quantal release, suggesting variability in fusion pore dynamics controlling the glutamate release by cells in this brain region. Hence, this novel biosensor allows recording of rapid single glutamate exocytosis events in the brain tissue and offers insight on regulatory aspects of exocytotic glutamate release, which is critical to understanding of brain glutamate function and dysfunction.

microelectrode

rodent

fusion pore

amperometry

glutamate oxidase

brain slice

nucleus accumbens

gold nanoparticle

ultrafast

biosensor

Glutamate

exocytosis

dynamics

Author

Yuanmo Wang

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Physical Chemistry

Devesh Mishra

Wallenberg Lab.

Sahlgrenska Academy

Jenny Bergman

University of Gothenburg

Jacqueline Keighron

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Analytical Chemistry

Karolina P. Skibicka

Sahlgrenska Academy

Wallenberg Lab.

Ann-Sofie Cans

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

ACS Chemical Neuroscience

1948-7193 (ISSN)

Vol. 10 3 1744-1752

Subject Categories

Cell Biology

Other Medical Engineering

Neurosciences

DOI

10.1021/acschemneuro.8b00624

PubMed

30605606

More information

Latest update

4/29/2019