Development of an Organic Lateral Resolution Test Device for Imaging Mass Spectrometry
Artikel i vetenskaplig tidskrift, 2014

An organic lateral resolution test device has been developed to measure the performance of imaging mass spectrometry (IMS) systems. The device contains periodic gratings of polyethylene glycol (PEG) and lipid bars covering a wide range of spatial frequencies. Microfabrication technologies were employed to produce well-defined chemical interfaces, which allow lateral resolution to be assessed using the edge-spread function (ESF). In addition, the design of the device allows for the direct measurement of the modulation transfer function (MTF) to assess image quality. Scanning electron microscopy (SEM) and time-of-flight secondary ion mass spectrometry (TOF-SIMS) were used to characterize the device. TOF-SIMS imaging was used to measure the chemical displacement of biomolecules in matrix-assisted laser desorption/ionization (MALDI) matrix crystals. In a proof-of-concept experiment, the platform was also used to evaluate MALDI matrix application methods, specifically aerosol spray and sublimation methods.

Författare

Melissa Passarelli

Chalmers, Kemi- och bioteknik

Göteborgs universitet

Jun Wang

Göteborgs universitet

Chalmers, Kemi- och bioteknik, Analytisk kemi

Amir Saeid Mohammadi

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

R. Trouillon

Göteborgs universitet

I. Gilmore

National Physical Laboratory (NPL)

Andrew Ewing

Göteborgs universitet

Chalmers, Kemi- och bioteknik, Analytisk kemi

Analytical Chemistry

0003-2700 (ISSN) 1520-6882 (eISSN)

Vol. 86 19 9473-9480

Ämneskategorier

Analytisk kemi

DOI

10.1021/ac501228x