Development of an Organic Lateral Resolution Test Device for Imaging Mass Spectrometry
Journal article, 2014

An organic lateral resolution test device has been developed to measure the performance of imaging mass spectrometry (IMS) systems. The device contains periodic gratings of polyethylene glycol (PEG) and lipid bars covering a wide range of spatial frequencies. Microfabrication technologies were employed to produce well-defined chemical interfaces, which allow lateral resolution to be assessed using the edge-spread function (ESF). In addition, the design of the device allows for the direct measurement of the modulation transfer function (MTF) to assess image quality. Scanning electron microscopy (SEM) and time-of-flight secondary ion mass spectrometry (TOF-SIMS) were used to characterize the device. TOF-SIMS imaging was used to measure the chemical displacement of biomolecules in matrix-assisted laser desorption/ionization (MALDI) matrix crystals. In a proof-of-concept experiment, the platform was also used to evaluate MALDI matrix application methods, specifically aerosol spray and sublimation methods.

Author

Melissa Passarelli

Chalmers, Chemical and Biological Engineering

University of Gothenburg

Jun Wang

University of Gothenburg

Chalmers, Chemical and Biological Engineering, Analytical Chemistry

Amir Saeid Mohammadi

Chalmers, Chemical and Biological Engineering, Physical Chemistry

R. Trouillon

University of Gothenburg

I. Gilmore

National Physical Laboratory (NPL)

Andrew Ewing

University of Gothenburg

Chalmers, Chemical and Biological Engineering, Analytical Chemistry

Analytical Chemistry

0003-2700 (ISSN) 1520-6882 (eISSN)

Vol. 86 19 9473-9480

Subject Categories

Analytical Chemistry

DOI

10.1021/ac501228x

More information

Latest update

5/29/2018